Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5396754..5397379 Replicon chromosome
Accession NZ_CP087623
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH50_RS26670 Protein ID WP_002882817.1
Coordinates 5396754..5397137 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH50_RS26675 Protein ID WP_004150355.1
Coordinates 5397137..5397379 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH50_RS26655 (LMH50_26655) 5394120..5395022 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH50_RS26660 (LMH50_26660) 5395019..5395654 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH50_RS26665 (LMH50_26665) 5395651..5396580 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH50_RS26670 (LMH50_26670) 5396754..5397137 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH50_RS26675 (LMH50_26675) 5397137..5397379 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH50_RS26680 (LMH50_26680) 5397584..5398501 + 918 WP_002882812.1 alpha/beta hydrolase -
LMH50_RS26685 (LMH50_26685) 5398515..5399456 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH50_RS26690 (LMH50_26690) 5399501..5399938 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH50_RS26695 (LMH50_26695) 5399935..5400795 - 861 WP_002882807.1 virulence factor BrkB family protein -
LMH50_RS26700 (LMH50_26700) 5400789..5401388 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225719 WP_002882817.1 NZ_CP087623:c5397137-5396754 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225719 NZ_CP121295:c4189166-4188879 [Salmonella enterica subsp. enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCTGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225719 WP_004150355.1 NZ_CP087623:c5397379-5397137 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225719 NZ_CP121295:c4188892-4188590 [Salmonella enterica subsp. enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGTGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References