Detailed information of TA system TA022571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1073449..1074049
• Replicon: chromosome
• Accession: NC_010125
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A9HCY6
• Locus tag: GDI_RS05090
• Protein ID: WP_012223960.1
• Coordinates: 1073705..1074049 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5YEQ9
• Locus tag: GDI_RS05085
• Protein ID: WP_010511780.1
• Coordinates: 1073449..1073712 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDI_RS05050	1068580..1069029	-	450	Protein_1000	IS5-like element ISGdi1 family transposase	-
GDI_RS05055	1069166..1069723	+	558	WP_012223947.1	phosphate-starvation-inducible PsiE family protein	-
GDI_RS05065	1070105..1071332	+	1228	Protein_1002	IS3-like element ISGdi14 family transposase	-
GDI_RS05070	1071510..1071992	-	483	WP_012223951.1	Hsp20 family protein	-
GDI_RS05075	1072028..1072309	-	282	WP_039905436.1	hypothetical protein	-
GDI_RS20140	1072479..1072571	+	93	WP_197535947.1	YXWGXW repeat-containing protein	-
GDI_RS05080	1073092..1073340	+	249	WP_010511782.1	hypothetical protein	-
GDI_RS05085	1073449..1073712	+	264	WP_010511780.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GDI_RS05090	1073705..1074049	+	345	WP_012223960.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GDI_RS05095	1074082..1074759	+	678	Protein_1009	recombinase family protein	-
GDI_RS18915	1074987..1075187	+	201	Protein_1010	IS630 family transposase	-
GDI_RS05100	1075224..1075685	+	462	Protein_1011	IS256 family transposase	-
GDI_RS05105	1075734..1076096	-	363	Protein_1012	helix-turn-helix domain-containing protein	-
GDI_RS05115	1076208..1077325	+	1118	WP_157870996.1	IS3 family transposase	-
GDI_RS05120	1077462..1078673	-	1212	WP_041249691.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1050440..1098494	48054
	-	inside	IScluster/Tn	-	-	1058482..1095203	36721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13319.12 Da
• Isoelectric Point: 9.9550

>T22571 WP_012223960.1 NC_010125:1073705-1074049 [Gluconacetobacter diazotrophicus PA1 5]
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAV
GRREDNEIYTAATGRIPETPADRTRQPSSRSRKR

Nucleotide

• Length: 345 bp

>T22571 NC_010125:1073705-1074049 [Gluconacetobacter diazotrophicus PA1 5]
ATGACTGAATACGCGATCGAATTCGATGATCGCGCACTTCGGGAATGGGACGGGCTTGACGGCAGCATTCGCAGGAAGTT
CGAGAAAAAGCTCGAAAAACTGGTTCTGAACCCGCATTCTCCCGGTAACGAACTGCACGGCGATCTCGCGGGATTCTACA
AGATCAAGCTGCGGCAGGACGGCTATCGCCTTGTCTATCAGGTCGTGGAACAGCGGATCGTCATCTTCGTCATCGCGGTC
GGACGCCGCGAGGACAACGAAATCTACACGGCGGCAACCGGGCGCATCCCGGAGACGCCAGCCGATCGGACACGTCAGCC
CAGCAGCAGAAGCCGCAAGAGGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9629.04 Da
• Isoelectric Point: 7.3200

>AT22571 WP_010511780.1 NC_010125:1073449-1073712 [Gluconacetobacter diazotrophicus PA1 5]
MSAVTFRVDDALKSAAVAKLSAHGLSLSDVLRDTLAYIAETGQPPVKRRLVTDEDARLIEIVRERLADPAPRHRMTLAEL
KARHPDD

Nucleotide

• Length: 264 bp

>AT22571 NC_010125:1073449-1073712 [Gluconacetobacter diazotrophicus PA1 5]
ATGTCCGCCGTGACGTTCCGTGTCGATGACGCCCTCAAATCCGCCGCTGTCGCCAAACTCTCCGCGCACGGCCTGTCTCT
CTCCGACGTCCTGCGCGATACCCTGGCCTATATCGCCGAGACCGGCCAGCCACCGGTCAAGCGGCGGCTTGTGACCGACG
AAGATGCAAGGCTGATCGAAATCGTCCGAGAACGCCTCGCCGATCCCGCCCCCCGCCACCGCATGACGCTGGCGGAACTC
AAGGCGCGCCATCCGGATGACTGA

Structures

Toxin

• AlphaFold DB: A9HCY6

Antitoxin

• AlphaFold DB: A0A7W4J448