Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 876230..876884 Replicon chromosome
Accession NZ_CP087623
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02280

Toxin (Protein)


Gene name cptA Uniprot ID R4YHX2
Locus tag LMH50_RS04410 Protein ID WP_004144731.1
Coordinates 876477..876884 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag LMH50_RS04405 Protein ID WP_002916312.1
Coordinates 876230..876496 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH50_RS04380 (LMH50_04380) 871386..872819 - 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -
LMH50_RS04385 (LMH50_04385) 872938..873666 - 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
LMH50_RS04390 (LMH50_04390) 873716..874027 + 312 WP_002916319.1 N(4)-acetylcytidine aminohydrolase -
LMH50_RS04395 (LMH50_04395) 874191..874850 + 660 WP_002916317.1 hemolysin III family protein -
LMH50_RS04400 (LMH50_04400) 875001..875984 - 984 WP_002916313.1 tRNA-modifying protein YgfZ -
LMH50_RS04405 (LMH50_04405) 876230..876496 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
LMH50_RS04410 (LMH50_04410) 876477..876884 + 408 WP_004144731.1 protein YgfX Toxin
LMH50_RS04415 (LMH50_04415) 876891..877412 - 522 WP_002916308.1 flavodoxin FldB -
LMH50_RS04420 (LMH50_04420) 877513..878409 + 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
LMH50_RS04425 (LMH50_04425) 878432..879145 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LMH50_RS04430 (LMH50_04430) 879151..880884 + 1734 WP_004151783.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15936.69 Da        Isoelectric Point: 11.4778

>T225705 WP_004144731.1 NZ_CP087623:876477-876884 [Klebsiella pneumoniae subsp. pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 408 bp

>T225705 NZ_CP121294:c3030513-3030340 [Escherichia coli O155:H21]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT225705 WP_002916312.1 NZ_CP087623:876230-876496 [Klebsiella pneumoniae subsp. pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225705 NZ_CP121294:c3030837-3030541 [Escherichia coli O155:H21]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378E4P6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References