Detailed information of TA system TA225701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 5074..5654
• Replicon: plasmid pDD02162-4
• Accession: NZ_CP087622
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LMH58_RS27315
• Protein ID: WP_071177730.1
• Coordinates: 5340..5654 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LMH58_RS27310
• Protein ID: WP_000093040.1
• Coordinates: 5074..5352 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH58_RS27280 (LMH58_27280)	1134..1283	-	150	WP_032440454.1	colicin release lysis protein	-
LMH58_RS27285 (LMH58_27285)	1368..1625	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
LMH58_RS27290 (LMH58_27290)	1635..3320	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
LMH58_RS27295 (LMH58_27295)	3647..3892	+	246	WP_032440458.1	hypothetical protein	-
LMH58_RS27300 (LMH58_27300)	4166..4537	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
LMH58_RS27305 (LMH58_27305)	4534..4899	+	366	WP_072354022.1	TonB family protein	-
LMH58_RS27310 (LMH58_27310)	5074..5352	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LMH58_RS27315 (LMH58_27315)	5340..5654	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH58_RS27320 (LMH58_27320)	5818..6246	+	429	WP_001140599.1	hypothetical protein	-
LMH58_RS27325 (LMH58_27325)	6272..6451	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LMH58_RS27330 (LMH58_27330)	6478..7008	-	531	WP_071177729.1	hypothetical protein	-
LMH58_RS27335 (LMH58_27335)	7015..7746	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
LMH58_RS27340 (LMH58_27340)	7746..9710	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225701 WP_071177730.1 NZ_CP087622:5340-5654 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225701 NZ_CP121294:2249184-2249534 [Escherichia coli O155:H21]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225701 WP_000093040.1 NZ_CP087622:5074-5352 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225701 NZ_CP121294:2248939-2249190 [Escherichia coli O155:H21]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1