Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30095..30738 Replicon plasmid pDD02162-3
Accession NZ_CP087621
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag LMH58_RS27125 Protein ID WP_000754566.1
Coordinates 30095..30511 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag LMH58_RS27130 Protein ID WP_001261276.1
Coordinates 30508..30738 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH58_RS27100 (LMH58_27100) 25960..26046 + 87 Protein_32 Arm DNA-binding domain-containing protein -
LMH58_RS27105 (LMH58_27105) 26318..27073 - 756 Protein_33 IS110-like element IS5075 family transposase -
LMH58_RS27110 (LMH58_27110) 27126..27830 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LMH58_RS27115 (LMH58_27115) 27893..28753 - 861 Protein_35 IS110 family transposase -
LMH58_RS27120 (LMH58_27120) 29035..29891 - 857 Protein_36 IS3-like element ISEc15 family transposase -
LMH58_RS27125 (LMH58_27125) 30095..30511 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH58_RS27130 (LMH58_27130) 30508..30738 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LMH58_RS27135 (LMH58_27135) 31327..31620 + 294 WP_020956883.1 hypothetical protein -
LMH58_RS27140 (LMH58_27140) 31684..32370 + 687 WP_020956882.1 hypothetical protein -
LMH58_RS27145 (LMH58_27145) 32382..33158 + 777 WP_020956881.1 tyrosine-type recombinase/integrase -
LMH58_RS27150 (LMH58_27150) 33216..33473 - 258 WP_000764642.1 hypothetical protein -
LMH58_RS27155 (LMH58_27155) 33602..33706 - 105 WP_032409716.1 hypothetical protein -
LMH58_RS27160 (LMH58_27160) 34236..35102 + 867 WP_004118283.1 replication initiation protein -
LMH58_RS27165 (LMH58_27165) 35279..35548 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / aac(3)-IId / aadA5 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / catA2 - 1..60051 60051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T225700 WP_000754566.1 NZ_CP087621:c30511-30095 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T225700 NZ_CP121294:c1867630-1867223 [Escherichia coli O155:H21]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT225700 WP_001261276.1 NZ_CP087621:c30738-30508 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT225700 NZ_CP121294:c1867877-1867611 [Escherichia coli O155:H21]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References