Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 53667..54403 | Replicon | plasmid pDD02162-1 |
| Accession | NZ_CP087619 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD02162 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | L7SZ15 |
| Locus tag | LMH58_RS25650 | Protein ID | WP_003026803.1 |
| Coordinates | 53921..54403 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | L7SZ29 |
| Locus tag | LMH58_RS25645 | Protein ID | WP_003026799.1 |
| Coordinates | 53667..53933 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH58_RS25600 (LMH58_25600) | 49729..50091 | - | 363 | WP_004152100.1 | arsenite efflux transporter metallochaperone ArsD | - |
| LMH58_RS25605 (LMH58_25605) | 50141..50491 | - | 351 | WP_004152101.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| LMH58_RS25610 (LMH58_25610) | 50849..51118 | + | 270 | WP_004152102.1 | hypothetical protein | - |
| LMH58_RS25615 (LMH58_25615) | 51106..51681 | + | 576 | WP_004152103.1 | hypothetical protein | - |
| LMH58_RS25620 (LMH58_25620) | 51712..52206 | + | 495 | WP_004152104.1 | DNA-binding protein | - |
| LMH58_RS25625 (LMH58_25625) | 52250..52618 | + | 369 | WP_004152105.1 | hypothetical protein | - |
| LMH58_RS25630 (LMH58_25630) | 52652..52855 | + | 204 | WP_004152106.1 | HHA domain-containing protein | - |
| LMH58_RS25635 (LMH58_25635) | 52904..53161 | + | 258 | WP_004152107.1 | hypothetical protein | - |
| LMH58_RS25640 (LMH58_25640) | 53237..53491 | + | 255 | WP_004152108.1 | hypothetical protein | - |
| LMH58_RS25645 (LMH58_25645) | 53667..53933 | + | 267 | WP_003026799.1 | DUF1778 domain-containing protein | Antitoxin |
| LMH58_RS25650 (LMH58_25650) | 53921..54403 | + | 483 | WP_003026803.1 | GNAT family N-acetyltransferase | Toxin |
| LMH58_RS25655 (LMH58_25655) | 54615..55961 | + | 1347 | WP_020314316.1 | ISNCY family transposase | - |
| LMH58_RS27380 | 56122..56253 | + | 132 | WP_004218042.1 | hypothetical protein | - |
| LMH58_RS25660 (LMH58_25660) | 56462..57627 | - | 1166 | Protein_59 | IS3 family transposase | - |
| LMH58_RS25665 (LMH58_25665) | 57804..58766 | - | 963 | WP_004152113.1 | zinc metalloprotease HtpX | - |
| LMH58_RS25670 (LMH58_25670) | 58753..59241 | - | 489 | WP_004152114.1 | phosphate-starvation-inducible PsiE family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(3')-Ia / mph(A) / sul1 / blaDHA-1 / qnrB4 | - | 1..209653 | 209653 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17353.97 Da Isoelectric Point: 9.5822
>T225699 WP_003026803.1 NZ_CP087619:53921-54403 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
Download Length: 483 bp
>T225699 NZ_CP121294:c1731295-1730960 [Escherichia coli O155:H21]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG
Antitoxin
Download Length: 89 a.a. Molecular weight: 10147.59 Da Isoelectric Point: 4.5256
>AT225699 WP_003026799.1 NZ_CP087619:53667-53933 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV
Download Length: 267 bp
>AT225699 NZ_CP121294:c1731543-1731295 [Escherichia coli O155:H21]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2GNW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YL66 |