Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5201165..5201790 Replicon chromosome
Accession NZ_CP087618
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH58_RS25005 Protein ID WP_002882817.1
Coordinates 5201165..5201548 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH58_RS25010 Protein ID WP_004150355.1
Coordinates 5201548..5201790 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH58_RS24990 (5198531) 5198531..5199433 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH58_RS24995 (5199430) 5199430..5200065 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH58_RS25000 (5200062) 5200062..5200991 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH58_RS25005 (5201165) 5201165..5201548 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH58_RS25010 (5201548) 5201548..5201790 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH58_RS25015 (5201995) 5201995..5202912 + 918 WP_004181612.1 alpha/beta hydrolase -
LMH58_RS25020 (5202926) 5202926..5203867 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH58_RS25025 (5203912) 5203912..5204349 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH58_RS25030 (5204346) 5204346..5205206 - 861 WP_004146232.1 virulence factor BrkB family protein -
LMH58_RS25035 (5205200) 5205200..5205799 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225698 WP_002882817.1 NZ_CP087618:c5201548-5201165 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225698 NZ_CP121294:c1517368-1517150 [Escherichia coli O155:H21]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225698 WP_004150355.1 NZ_CP087618:c5201790-5201548 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225698 NZ_CP121294:c1517768-1517394 [Escherichia coli O155:H21]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References