Detailed information of TA system TA225695

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4642855..4643690
• Replicon: chromosome
• Accession: NZ_CP087618
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5E2RWI0
• Locus tag: LMH58_RS22405
• Protein ID: WP_001559913.1
• Coordinates: 4642855..4643232 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A5E3EVB5
• Locus tag: LMH58_RS22410
• Protein ID: WP_032408847.1
• Coordinates: 4643322..4643690 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH58_RS22365 (4638120)	4638120..4638386	+	267	WP_004178415.1	hypothetical protein	-
LMH58_RS22370 (4638386)	4638386..4639058	+	673	Protein_4387	DUF4400 domain-containing protein	-
LMH58_RS22375 (4639069)	4639069..4639785	+	717	WP_223203127.1	RES domain-containing protein	-
LMH58_RS22380 (4640153)	4640153..4640341	-	189	Protein_4389	transposase	-
LMH58_RS22385 (4640358)	4640358..4641469	-	1112	Protein_4390	IS3 family transposase	-
LMH58_RS22390 (4641919)	4641919..4642068	-	150	Protein_4391	restriction endonuclease subunit M	-
LMH58_RS22395 (4642153)	4642153..4642350	-	198	WP_032408848.1	DUF957 domain-containing protein	-
LMH58_RS22400 (4642370)	4642370..4642858	-	489	WP_001559914.1	DUF5983 family protein	-
LMH58_RS22405 (4642855)	4642855..4643232	-	378	WP_001559913.1	TA system toxin CbtA family protein	Toxin
LMH58_RS22410 (4643322)	4643322..4643690	-	369	WP_032408847.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LMH58_RS22415 (4643867)	4643867..4644088	-	222	WP_000692300.1	DUF987 domain-containing protein	-
LMH58_RS22420 (4644157)	4644157..4644633	-	477	WP_032408846.1	RadC family protein	-
LMH58_RS22425 (4644648)	4644648..4645133	-	486	WP_032408845.1	antirestriction protein	-
LMH58_RS22430 (4645225)	4645225..4646043	-	819	WP_001559911.1	DUF932 domain-containing protein	-
LMH58_RS22435 (4646143)	4646143..4646376	-	234	WP_001119729.1	DUF905 family protein	-
LMH58_RS22440 (4646455)	4646455..4646910	-	456	WP_000581504.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4635359..4662712	27353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.10 Da
• Isoelectric Point: 7.8276

>T225695 WP_001559913.1 NZ_CP087618:c4643232-4642855 [Klebsiella pneumoniae subsp. pneumoniae]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T225695 NZ_CP121294:c1176891-1176697 [Escherichia coli O155:H21]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13682.62 Da
• Isoelectric Point: 7.8398

>AT225695 WP_032408847.1 NZ_CP087618:c4643690-4643322 [Klebsiella pneumoniae subsp. pneumoniae]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVMLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT225695 NZ_CP121294:1176520-1176698 [Escherichia coli O155:H21]
GAGGACTGAAGTTTCTCGCAATTAAAATTAATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A5E2RWI0

Antitoxin

• AlphaFold DB: A0A5E3EVB5