Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4603481..4604291 Replicon chromosome
Accession NZ_CP087618
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LMH58_RS22205 Protein ID WP_004178461.1
Coordinates 4603481..4604014 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH58_RS22210 Protein ID WP_002887278.1
Coordinates 4604025..4604291 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH58_RS22200 (4602312) 4602312..4603433 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH58_RS22205 (4603481) 4603481..4604014 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LMH58_RS22210 (4604025) 4604025..4604291 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH58_RS22215 (4604394) 4604394..4605827 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH58_RS22220 (4605817) 4605817..4606500 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH58_RS22225 (4606672) 4606672..4608057 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
LMH58_RS22230 (4608075) 4608075..4608419 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T225694 WP_004178461.1 NZ_CP087618:c4604014-4603481 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225694 NZ_CP121294:c1039394-1039287 [Escherichia coli O155:H21]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225694 WP_002887278.1 NZ_CP087618:c4604291-4604025 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225694 NZ_CP121294:1039441-1039507 [Escherichia coli O155:H21]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References