Detailed information of TA system TA225691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2640880..2642468
• Replicon: chromosome
• Accession: NZ_CP087618
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02162

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LMH58_RS12805
• Protein ID: WP_004179815.1
• Coordinates: 2640880..2642202 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LMH58_RS12810
• Protein ID: WP_004176198.1
• Coordinates: 2642202..2642468 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH58_RS12790 (2635936)	2635936..2638437	+	2502	WP_020323802.1	fimbria/pilus outer membrane usher protein	-
LMH58_RS12795 (2638425)	2638425..2639429	+	1005	WP_004179822.1	fimbrial protein	-
LMH58_RS12800 (2639571)	2639571..2640863	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LMH58_RS12805 (2640880)	2640880..2642202	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
LMH58_RS12810 (2642202)	2642202..2642468	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LMH58_RS12815 (2642734)	2642734..2643060	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LMH58_RS12820 (2643057)	2643057..2643557	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LMH58_RS12825 (2643535)	2643535..2644914	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
LMH58_RS12830 (2645260)	2645260..2646414	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LMH58_RS12835 (2646395)	2646395..2647318	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC	2473216..2677303	204087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T225691 WP_004179815.1 NZ_CP087618:c2642202-2640880 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T225691 NZ_CP121294:c475956-475702 [Escherichia coli O155:H21]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT225691 WP_004176198.1 NZ_CP087618:c2642468-2642202 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT225691 NZ_CP121294:c476204-475953 [Escherichia coli O155:H21]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA