Detailed information of TA system TA022568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 70757..71391
• Replicon: chromosome
• Accession: NC_010125
• Organism: Gluconacetobacter diazotrophicus PAl 5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GDI_RS00295
• Protein ID: WP_012222312.1
• Coordinates: 70981..71391 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A9GZV6
• Locus tag: GDI_RS00290
• Protein ID: WP_012222311.1
• Coordinates: 70757..70981 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GDI_RS00270	66416..67072	-	657	WP_012222307.1	CRISPR-associated protein Cas4	-
GDI_RS00275	67081..68019	-	939	WP_012553888.1	type I-C CRISPR-associated protein Cas7/Csd2	-
GDI_RS00280	68012..69835	-	1824	WP_012222309.1	type I-C CRISPR-associated protein Cas8c/Csd1	-
GDI_RS00285	69832..70506	-	675	WP_197535950.1	type I-C CRISPR-associated protein Cas5	-
GDI_RS00290	70757..70981	+	225	WP_012222311.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GDI_RS00295	70981..71391	+	411	WP_012222312.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GDI_RS00300	71552..73900	-	2349	WP_012222313.1	CRISPR-associated endonuclease Cas3''	-
GDI_RS00305	73931..74311	-	381	WP_041249211.1	histidine triad nucleotide-binding protein	-
GDI_RS00310	74335..74862	-	528	WP_012222315.1	phosphoribosyl-ATP diphosphatase	-
GDI_RS00315	74904..75680	-	777	WP_012222316.1	imidazole glycerol phosphate synthase subunit HisF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15419.60 Da
• Isoelectric Point: 6.7075

>T22568 WP_012222312.1 NC_010125:70981-71391 [Gluconacetobacter diazotrophicus PA1 5]
MLRFLLDTNLCIRVLRDRPQGLRARFNAYAEDLCISDVVLYELLYGAERSSDPVRVRREVEHFADRLNVLPFDSAAASHT
AEIRDDLERRGCVIGPYDLMIAGHARSRGLVVVTGNLGEFRRVAGLRAEDWFTNVA

Nucleotide

• Length: 411 bp

>T22568 NC_010125:70981-71391 [Gluconacetobacter diazotrophicus PA1 5]
ATGCTGCGCTTTCTGCTGGACACCAACTTGTGCATCCGTGTGTTGCGGGACCGGCCGCAGGGCCTGCGGGCGCGGTTCAA
TGCCTACGCCGAAGACCTCTGTATCTCCGATGTCGTGCTATACGAACTGCTCTATGGGGCGGAGCGGTCGTCCGATCCCG
TGCGTGTCAGGCGAGAGGTCGAGCATTTCGCGGACAGACTGAATGTCCTGCCGTTCGACAGCGCGGCGGCGTCCCATACG
GCCGAGATCCGGGACGATCTCGAACGCAGGGGCTGCGTGATCGGCCCCTATGATCTGATGATCGCGGGACACGCCCGCAG
CCGGGGGTTGGTGGTCGTCACAGGCAATCTGGGCGAGTTCAGGCGCGTTGCGGGGCTCAGGGCCGAAGACTGGTTCACGA
ATGTTGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8240.46 Da
• Isoelectric Point: 6.2937

>AT22568 WP_012222311.1 NC_010125:70757-70981 [Gluconacetobacter diazotrophicus PA1 5]
MLTRTTLFLSNRSQAVRLPKAVAFGEGVREVVIVPEGPRRIIAPAEAAWDDFFAAPGLDLGERSQPAMQERERF

Nucleotide

• Length: 225 bp

>AT22568 NC_010125:70757-70981 [Gluconacetobacter diazotrophicus PA1 5]
ATGCTCACGCGCACGACCTTGTTTCTCTCCAATCGTTCCCAGGCGGTTCGCCTGCCGAAGGCCGTGGCCTTTGGAGAAGG
CGTGCGGGAGGTGGTGATTGTTCCCGAAGGCCCGCGTCGGATTATCGCGCCCGCTGAAGCAGCCTGGGACGATTTTTTCG
CCGCTCCCGGCCTGGACCTGGGGGAACGCAGCCAGCCGGCGATGCAGGAACGGGAGCGCTTCTGA

Structures

Antitoxin

• AlphaFold DB: A9GZV6