Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1904..2481 Replicon plasmid pDD02172-3
Accession NZ_CP087614
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag LMH59_RS27730 Protein ID WP_059065587.1
Coordinates 2149..2481 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag LMH59_RS27725 Protein ID WP_032454911.1
Coordinates 1904..2149 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH59_RS27725 (LMH59_27725) 1904..2149 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LMH59_RS27730 (LMH59_27730) 2149..2481 + 333 WP_059065587.1 endoribonuclease MazF Toxin
LMH59_RS27735 (LMH59_27735) 2741..3064 + 324 WP_021312689.1 hypothetical protein -
LMH59_RS27740 (LMH59_27740) 3315..4019 - 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
LMH59_RS27745 (LMH59_27745) 4179..5525 - 1347 WP_188201154.1 ISNCY family transposase -
LMH59_RS27750 (LMH59_27750) 5738..5992 - 255 WP_065810046.1 hypothetical protein -
LMH59_RS27755 (LMH59_27755) 6068..6325 - 258 WP_047665813.1 hypothetical protein -
LMH59_RS27760 (LMH59_27760) 6374..6577 - 204 WP_004152106.1 HHA domain-containing protein -
LMH59_RS27765 (LMH59_27765) 6611..6979 - 369 WP_122462932.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..81442 81442
- flank IS/Tn - - 4179..5537 1358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T225677 WP_059065587.1 NZ_CP087614:2149-2481 [Klebsiella pneumoniae subsp. pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T225677 NZ_CP121269:c2302290-2301805 [Enterobacter hormaechei subsp. xiangfangensis]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAGCGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGAGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAACGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT225677 WP_032454911.1 NZ_CP087614:1904-2149 [Klebsiella pneumoniae subsp. pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT225677 NZ_CP121269:c2302544-2302278 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References