Detailed information of TA system TA225676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7009..7652
• Replicon: plasmid pDD02172-2
• Accession: NZ_CP087613
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: LMH59_RS27115
• Protein ID: WP_001044770.1
• Coordinates: 7236..7652 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LMH59_RS27110
• Protein ID: WP_001261282.1
• Coordinates: 7009..7239 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH59_RS27070 (2182)	2182..2484	+	303	WP_004197636.1	hypothetical protein	-
LMH59_RS27075 (2972)	2972..3766	-	795	WP_004197635.1	site-specific integrase	-
LMH59_RS27080 (3964)	3964..4980	-	1017	WP_017899884.1	hypothetical protein	-
LMH59_RS27085 (4991)	4991..5305	-	315	WP_053389906.1	hypothetical protein	-
LMH59_RS27090 (5332)	5332..5727	-	396	WP_017899885.1	hypothetical protein	-
LMH59_RS27095 (5896)	5896..6201	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
LMH59_RS27100 (6203)	6203..6421	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
LMH59_RS27105 (6591)	6591..7052	-	462	WP_160880186.1	hypothetical protein	-
LMH59_RS27110 (7009)	7009..7239	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LMH59_RS27115 (7236)	7236..7652	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMH59_RS27120 (7726)	7726..9288	+	1563	WP_004206609.1	AAA family ATPase	-
LMH59_RS27125 (9273)	9273..10295	+	1023	WP_000361404.1	helicase UvrD	-
LMH59_RS27130 (10551)	10551..11248	+	698	WP_223174630.1	IS1 family transposase	-
LMH59_RS27135 (11277)	11277..11549	+	273	Protein_15	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-187	-	1..106447	106447

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T225676 WP_001044770.1 NZ_CP087613:7236-7652 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T225676 NZ_CP121269:c1874772-1874593 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTGAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT225676 WP_001261282.1 NZ_CP087613:7009-7239 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT225676 NZ_CP121269:c1874572-1874162 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGATGATTATCAGGACGCGATTGAAAGCGTACGTGAAGCGATCGAGGCGCATATTGAATTACTGGTTGAAGATGGCG
AGGCCGTACCCGAAGCAACGACCGTCGAAAACTGGCTTTCTGATCCGGAGTACGCCGGTGCGGTATGGGCCTTGGTTGAC
GTGGATATAACTCGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTTCCCTCATTACTGATCCGTCGTATCGATCA
GTTTGTGGCCGCGCATCCAGAATACGGCAGTCGGTCTGGCTTCTTATCGCGCGTCGCGGCAGATAAAGTAATTGGACGAG
AAAAACGATAG

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3