Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 5896..6421 | Replicon | plasmid pDD02172-2 |
| Accession | NZ_CP087613 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae strain DD02172 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | D4HQE7 |
| Locus tag | LMH59_RS27095 | Protein ID | WP_013023785.1 |
| Coordinates | 5896..6201 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | W8V2V6 |
| Locus tag | LMH59_RS27100 | Protein ID | WP_001568025.1 |
| Coordinates | 6203..6421 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LMH59_RS27065 (1559) | 1559..2185 | + | 627 | WP_004197649.1 | ParA family plasmid-partitioning AAA ATPase | - |
| LMH59_RS27070 (2182) | 2182..2484 | + | 303 | WP_004197636.1 | hypothetical protein | - |
| LMH59_RS27075 (2972) | 2972..3766 | - | 795 | WP_004197635.1 | site-specific integrase | - |
| LMH59_RS27080 (3964) | 3964..4980 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| LMH59_RS27085 (4991) | 4991..5305 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| LMH59_RS27090 (5332) | 5332..5727 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| LMH59_RS27095 (5896) | 5896..6201 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| LMH59_RS27100 (6203) | 6203..6421 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| LMH59_RS27105 (6591) | 6591..7052 | - | 462 | WP_160880186.1 | hypothetical protein | - |
| LMH59_RS27110 (7009) | 7009..7239 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| LMH59_RS27115 (7236) | 7236..7652 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | - |
| LMH59_RS27120 (7726) | 7726..9288 | + | 1563 | WP_004206609.1 | AAA family ATPase | - |
| LMH59_RS27125 (9273) | 9273..10295 | + | 1023 | WP_000361404.1 | helicase UvrD | - |
| LMH59_RS27130 (10551) | 10551..11248 | + | 698 | WP_223174630.1 | IS1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaKPC-2 / blaSHV-187 | - | 1..106447 | 106447 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11615.30 Da Isoelectric Point: 6.4672
>T225675 WP_013023785.1 NZ_CP087613:c6201-5896 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T225675 NZ_CP121269:c1201683-1201372 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGTTC
TTCATCAAACACATCAGCACGCACGCAGAGTATGACAAGCTCACGGACTACTATCGGAGAACGAAAGAATGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT225675 WP_001568025.1 NZ_CP087613:c6421-6203 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT225675 NZ_CP121269:c1201375-1200956 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CTAAAATCGATAAGTACGAAAACGAGTCCTCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CTAAAATCGATAAGTACGAAAACGAGTCCTCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGCGACTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTGAACGGACAGCGCACGTTAACGCTGGATCATATGCGGGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GCGCATTTGTGGGGAATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZW6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J4ZZP8 |