Detailed information of TA system TA225674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 211299..211821
• Replicon: plasmid pDD02172-1
• Accession: NZ_CP087612
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: LMH59_RS26715
• Protein ID: WP_004181778.1
• Coordinates: 211537..211821 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: LMH59_RS26710
• Protein ID: WP_004181777.1
• Coordinates: 211299..211547 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH59_RS26690 (LMH59_26690)	206508..207329	-	822	WP_004181772.1	hypothetical protein	-
LMH59_RS26695 (LMH59_26695)	207391..207744	-	354	WP_004181774.1	hypothetical protein	-
LMH59_RS26700 (LMH59_26700)	207889..208875	-	987	WP_101992392.1	hypothetical protein	-
LMH59_RS26705 (LMH59_26705)	209209..211008	-	1800	WP_004181776.1	ATP-dependent helicase	-
LMH59_RS26710 (LMH59_26710)	211299..211547	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LMH59_RS26715 (LMH59_26715)	211537..211821	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH59_RS26720 (LMH59_26720)	211838..211939	-	102	Protein_237	IS200/IS605 family transposase	-
LMH59_RS26725 (LMH59_26725)	211975..213201	+	1227	WP_040209639.1	RNA-guided endonuclease TnpB family protein	-
LMH59_RS26730 (LMH59_26730)	213472..213696	-	225	Protein_239	transposase	-
LMH59_RS26735 (LMH59_26735)	213775..214203	-	429	WP_077257669.1	IS200/IS605 family transposase	-
LMH59_RS26740 (LMH59_26740)	214239..215426	+	1188	WP_040209642.1	RNA-guided endonuclease TnpB family protein	-
LMH59_RS26745 (LMH59_26745)	215471..215842	-	372	WP_040209644.1	hypothetical protein	-
LMH59_RS26750 (LMH59_26750)	215839..216183	-	345	WP_223811496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaDHA-1 / qnrB4 / blaSHV-187	iucA / iucB / iucC / iucD / iutA / rmpA	1..281138	281138

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T225674 WP_004181778.1 NZ_CP087612:211537-211821 [Klebsiella pneumoniae subsp. pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T225674 NZ_CP121269:c1107194-1106976 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT225674 WP_004181777.1 NZ_CP087612:211299-211547 [Klebsiella pneumoniae subsp. pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT225674 NZ_CP121269:c1107596-1107222 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A2J4R0U8