225671

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 32645..33555 Replicon plasmid pDD02172-1
Accession NZ_CP087612
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LMH59_RS25745 Protein ID WP_004026354.1
Coordinates 33085..33555 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag LMH59_RS25740 Protein ID WP_004181895.1
Coordinates 32645..33088 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH59_RS25725 (LMH59_25725) 29278..30765 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
LMH59_RS25730 (LMH59_25730) 30851..31273 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
LMH59_RS25735 (LMH59_25735) 31273..32544 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
LMH59_RS25740 (LMH59_25740) 32645..33088 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
LMH59_RS25745 (LMH59_25745) 33085..33555 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LMH59_RS25750 (LMH59_25750) 33665..33925 - 261 WP_004026352.1 hypothetical protein -
LMH59_RS25755 (LMH59_25755) 34496..35677 - 1182 WP_078207741.1 IS481 family transposase -
LMH59_RS25760 (LMH59_25760) 35825..37165 + 1341 WP_000589001.1 ISNCY family transposase -
LMH59_RS25765 (LMH59_25765) 37586..37897 + 312 Protein_46 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaDHA-1 / qnrB4 / blaSHV-187 iucA / iucB / iucC / iucD / iutA / rmpA 1..281138 281138


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T225671 WP_004026354.1 NZ_CP087612:33085-33555 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T225671 NZ_CP121269:c308701-308414 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT225671 WP_004181895.1 NZ_CP087612:32645-33088 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT225671 NZ_CP121269:c309010-308708 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
CTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCTGTGGGCGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References