Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5241667..5242292 Replicon chromosome
Accession NZ_CP087611
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH59_RS25170 Protein ID WP_002882817.1
Coordinates 5241667..5242050 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH59_RS25175 Protein ID WP_004150355.1
Coordinates 5242050..5242292 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH59_RS25155 (5239033) 5239033..5239935 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH59_RS25160 (5239932) 5239932..5240567 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH59_RS25165 (5240564) 5240564..5241493 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH59_RS25170 (5241667) 5241667..5242050 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH59_RS25175 (5242050) 5242050..5242292 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH59_RS25180 (5242497) 5242497..5243414 + 918 WP_004181612.1 alpha/beta hydrolase -
LMH59_RS25185 (5243428) 5243428..5244369 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH59_RS25190 (5244414) 5244414..5244851 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH59_RS25195 (5244848) 5244848..5245708 - 861 WP_004146232.1 virulence factor BrkB family protein -
LMH59_RS25200 (5245702) 5245702..5246301 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225670 WP_002882817.1 NZ_CP087611:c5242050-5241667 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225670 NZ_CP121266:c1360856-1360110 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225670 WP_004150355.1 NZ_CP087611:c5242292-5242050 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225670 NZ_CP121266:c1360110-1359940 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References