Detailed information of TA system TA225670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5241667..5242292
• Replicon: chromosome
• Accession: NZ_CP087611
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: LMH59_RS25170
• Protein ID: WP_002882817.1
• Coordinates: 5241667..5242050 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: LMH59_RS25175
• Protein ID: WP_004150355.1
• Coordinates: 5242050..5242292 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH59_RS25155 (5239033)	5239033..5239935	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
LMH59_RS25160 (5239932)	5239932..5240567	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
LMH59_RS25165 (5240564)	5240564..5241493	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
LMH59_RS25170 (5241667)	5241667..5242050	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
LMH59_RS25175 (5242050)	5242050..5242292	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
LMH59_RS25180 (5242497)	5242497..5243414	+	918	WP_004181612.1	alpha/beta hydrolase	-
LMH59_RS25185 (5243428)	5243428..5244369	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
LMH59_RS25190 (5244414)	5244414..5244851	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
LMH59_RS25195 (5244848)	5244848..5245708	-	861	WP_004146232.1	virulence factor BrkB family protein	-
LMH59_RS25200 (5245702)	5245702..5246301	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T225670 WP_002882817.1 NZ_CP087611:c5242050-5241667 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T225670 NZ_CP121266:c1360856-1360110 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAACTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCCTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT225670 WP_004150355.1 NZ_CP087611:c5242292-5242050 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT225670 NZ_CP121266:c1360110-1359940 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8

Antitoxin

• AlphaFold DB: A0A0H3GGU9