Detailed information of TA system TA225668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4771716..4772232
• Replicon: chromosome
• Accession: NZ_CP087611
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: LMH59_RS22930
• Protein ID: WP_004178374.1
• Coordinates: 4771716..4772000 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LMH59_RS22935
• Protein ID: WP_002886901.1
• Coordinates: 4771990..4772232 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH59_RS22905 (4767200)	4767200..4767463	-	264	WP_020324507.1	PTS system, lactose/cellobiose-specific IIB subunit	-
LMH59_RS22910 (4767593)	4767593..4767766	+	174	WP_032408826.1	hypothetical protein	-
LMH59_RS22915 (4767769)	4767769..4768512	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LMH59_RS22920 (4768869)	4768869..4771007	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
LMH59_RS22925 (4771248)	4771248..4771712	+	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LMH59_RS22930 (4771716)	4771716..4772000	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH59_RS22935 (4771990)	4771990..4772232	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LMH59_RS22940 (4772310)	4772310..4774220	-	1911	WP_004178373.1	PRD domain-containing protein	-
LMH59_RS22945 (4774243)	4774243..4775397	-	1155	WP_004178372.1	lactonase family protein	-
LMH59_RS22950 (4775464)	4775464..4776204	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T225668 WP_004178374.1 NZ_CP087611:c4772000-4771716 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T225668 NZ_CP121262:4688631-4688942 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT225668 WP_002886901.1 NZ_CP087611:c4772232-4771990 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT225668 NZ_CP121262:4688939-4689385 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A6THG1

Antitoxin

• AlphaFold DB: A0A0H3GLP0