Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4643840..4644650 Replicon chromosome
Accession NZ_CP087611
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LMH59_RS22360 Protein ID WP_004178461.1
Coordinates 4643840..4644373 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH59_RS22365 Protein ID WP_002887278.1
Coordinates 4644384..4644650 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH59_RS22355 (4642671) 4642671..4643792 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH59_RS22360 (4643840) 4643840..4644373 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LMH59_RS22365 (4644384) 4644384..4644650 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH59_RS22370 (4644753) 4644753..4646186 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH59_RS22375 (4646176) 4646176..4646859 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH59_RS22380 (4647031) 4647031..4648416 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
LMH59_RS22385 (4648434) 4648434..4648778 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T225666 WP_004178461.1 NZ_CP087611:c4644373-4643840 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225666 NZ_CP121262:c4293942-4293451 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225666 WP_002887278.1 NZ_CP087611:c4644650-4644384 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225666 NZ_CP121262:c4294232-4293939 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References