Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4010323..4010942 Replicon chromosome
Accession NZ_CP087611
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD02172

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH59_RS19365 Protein ID WP_002892050.1
Coordinates 4010724..4010942 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH59_RS19360 Protein ID WP_002892066.1
Coordinates 4010323..4010697 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH59_RS19350 (4005475) 4005475..4006668 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH59_RS19355 (4006691) 4006691..4009837 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LMH59_RS19360 (4010323) 4010323..4010697 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH59_RS19365 (4010724) 4010724..4010942 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH59_RS19370 (4011101) 4011101..4011667 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH59_RS19375 (4011639) 4011639..4011779 - 141 WP_004147370.1 hypothetical protein -
LMH59_RS19380 (4011800) 4011800..4012270 + 471 WP_002892026.1 YlaC family protein -
LMH59_RS19385 (4012245) 4012245..4013679 - 1435 Protein_3806 PLP-dependent aminotransferase family protein -
LMH59_RS19390 (4013780) 4013780..4014478 + 699 WP_002892021.1 GNAT family protein -
LMH59_RS19395 (4014475) 4014475..4014615 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH59_RS19400 (4014615) 4014615..4014878 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225665 WP_002892050.1 NZ_CP087611:4010724-4010942 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225665 NZ_CP121262:c4164880-4164596 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225665 WP_002892066.1 NZ_CP087611:4010323-4010697 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225665 NZ_CP121262:c4165112-4164870 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References