Detailed information of TA system TA022566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 941573..942132
• Replicon: chromosome
• Accession: NC_010103
• Organism: Brucella canis ATCC 23365

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BCAN_RS04545
• Protein ID: WP_004688321.1
• Coordinates: 941887..942132 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BCAN_RS04540
• Protein ID: WP_002964097.1
• Coordinates: 941573..941887 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCAN_RS04495	937262..937465	-	204	WP_004690812.1	hypothetical protein	-
BCAN_RS04500	937468..937683	-	216	WP_041594673.1	hypothetical protein	-
BCAN_RS04505	937680..937883	-	204	WP_002964088.1	hypothetical protein	-
BCAN_RS04510	937933..938652	+	720	WP_006072696.1	hypothetical protein	-
BCAN_RS04515	938649..938879	+	231	WP_002964091.1	hypothetical protein	-
BCAN_RS04520	938876..939151	+	276	WP_002964092.1	hypothetical protein	-
BCAN_RS04525	939174..939761	+	588	WP_004683738.1	hypothetical protein	-
BCAN_RS04530	939996..940707	+	712	Protein_886	porin family protein	-
BCAN_RS04535	941055..941225	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BCAN_RS04540	941573..941887	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BCAN_RS04545	941887..942132	-	246	WP_004688321.1	BrnT family toxin	Toxin
BCAN_RS04550	942265..942903	+	639	Protein_890	tRNA-guanine transglycosylase	-
BCAN_RS17385	942973..943730	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
BCAN_RS04565	943731..944504	+	774	WP_006132649.1	hypothetical protein	-
BCAN_RS04570	944639..946285	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	935490..949601	14111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T22566 WP_004688321.1 NC_010103:c942132-941887 [Brucella canis ATCC 23365]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T22566 NC_010103:c942132-941887 [Brucella canis ATCC 23365]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT22566 WP_002964097.1 NC_010103:c941887-941573 [Brucella canis ATCC 23365]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT22566 NC_010103:c941887-941573 [Brucella canis ATCC 23365]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA