Detailed information of TA system TA225651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 751..1331
• Replicon: plasmid pDD01304-3
• Accession: NZ_CP087609
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD01304

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LMH54_RS29380
• Protein ID: WP_071177730.1
• Coordinates: 1017..1331 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LMH54_RS29375
• Protein ID: WP_000093040.1
• Coordinates: 751..1029 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH54_RS29370 (LMH54_29370)	211..576	+	366	WP_072354022.1	TonB family protein	-
LMH54_RS29375 (LMH54_29375)	751..1029	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LMH54_RS29380 (LMH54_29380)	1017..1331	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMH54_RS29385 (LMH54_29385)	1495..1923	+	429	WP_001140599.1	hypothetical protein	-
LMH54_RS29390 (LMH54_29390)	1949..2128	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LMH54_RS29395 (LMH54_29395)	2155..2685	-	531	WP_071177729.1	hypothetical protein	-
LMH54_RS29400 (LMH54_29400)	2692..3423	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
LMH54_RS29405 (LMH54_29405)	3423..5387	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10062	10062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T225651 WP_071177730.1 NZ_CP087609:1017-1331 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T225651 NZ_CP121261:c3350226-3349951 [Achromobacter spanius]
ATGCTTCCTATCTTTTGGAGTGCTAGCGCACTCGATGATCTGGACGAGATCACCAACTACATCGCGGAATACGACGTGCA
TGCAGCCATCGGCATGCACGAGCTGATCGAAAACGCAGTGCACCCAGCCTCAGAACATCCGTACCTCTACCGCCCGGGCC
GTGTGCCAGGTACGCGGGAAATCGTGGCACATCCCAATTACATCCTGGTCTACGAAGTCCGAGCCGACCACATCGGGGTC
ATAGCTGTCATGCACGCCCGCCAGGAATACCCATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT225651 WP_000093040.1 NZ_CP087609:751-1029 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT225651 NZ_CP121261:c3350478-3350260 [Achromobacter spanius]
ATGAGCCCAACCCTCTCCCCTATCGTTTCGGAATTCGAAACCGAAGAGCAAGCGGTCAGTTATGACCGCTGGTTTCGTGC
GAAAGTACAGGCCAGCCGGGACGACCCGCGCCCGAGCATTCCGCACGATGCCGCGATGGCGCGTATCCGTGAAAAATTGG
CAAAAAAGCTGGCCGATCATGATGCAGCGCCAGCTACGCGGCGCAGTCGAGACCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1