Detailed information of TA system TA022565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 407871..408466
• Replicon: chromosome
• Accession: NC_010087
• Organism: Burkholderia multivorans ATCC 17616

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A2A4C4A8
• Locus tag: BMUL_RS28700
• Protein ID: WP_012218081.1
• Coordinates: 407871..408221 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A2A4C1X6
• Locus tag: BMUL_RS28705
• Protein ID: WP_012218082.1
• Coordinates: 408215..408466 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMUL_RS34055	402996..403385	-	390	WP_080512399.1	hypothetical protein	-
BMUL_RS28675	403406..403615	-	210	WP_038442273.1	hypothetical protein	-
BMUL_RS28680	403633..403992	-	360	WP_012218076.1	hypothetical protein	-
BMUL_RS35000	404011..404454	-	444	WP_143331194.1	hypothetical protein	-
BMUL_RS28685	404464..404718	-	255	WP_038442290.1	hypothetical protein	-
BMUL_RS34060	404774..405718	-	945	WP_012218077.1	hypothetical protein	-
BMUL_RS35005	405932..406501	-	570	WP_012218078.1	hypothetical protein	-
BMUL_RS35010	406525..406857	-	333	WP_096508413.1	hypothetical protein	-
BMUL_RS35015	406942..407103	+	162	WP_155295622.1	hypothetical protein	-
BMUL_RS28690	407100..407285	-	186	WP_038442441.1	hypothetical protein	-
BMUL_RS28695	407348..407860	-	513	WP_012218080.1	hypothetical protein	-
BMUL_RS28700	407871..408221	-	351	WP_012218081.1	type II toxin-antitoxin system ChpB family toxin	Toxin
BMUL_RS28705	408215..408466	-	252	WP_012218082.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BMUL_RS28710	408658..409338	-	681	WP_012218083.1	site-specific integrase	-
BMUL_RS28715	409503..409829	+	327	WP_012464905.1	H-NS histone family protein	-
BMUL_RS28720	409960..410751	+	792	WP_012218085.1	SOS response-associated peptidase family protein	-
BMUL_RS28725	410924..411118	-	195	WP_012464904.1	hypothetical protein	-
BMUL_RS28735	412011..412517	+	507	WP_012218086.1	arsenate reductase (azurin) small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-115)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12420.35 Da
• Isoelectric Point: 8.9716

>T22565 WP_012218081.1 NC_010087:c408221-407871 [Burkholderia multivorans ATCC 17616]
MVKRVKFDRGDIVRVSLNRTIGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEARGARTIERAPAEVVEDALARLQSIIE

Nucleotide

• Length: 351 bp

>T22565 NC_010087:c408221-407871 [Burkholderia multivorans ATCC 17616]
ATGGTAAAGCGGGTCAAGTTCGATCGGGGCGACATCGTGCGCGTTAGCCTGAACCGGACCATCGGGCGCGAGCAGCAGGG
CGATTTCCGTCCCGCGCTTGTGCTGTCTCCCGCCGCATTCAATGCCTTGGGGGTGGCGCTCGTCGCGCCGATCACGCAGG
GCGGGGAGTTTGCGCGCTTCGCAGGATTCGCCGTGCCGCTCTCTGGCTCGGGCACCGAGACGCAGGGCGTGGCCTTGGTG
AACATGGTCCGGATGCTCGATCTGGAGGCGCGTGGCGCACGGACGATCGAGCGGGCTCCGGCCGAGGTGGTCGAAGATGC
GCTGGCTCGGCTGCAATCGATAATCGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8997.39 Da
• Isoelectric Point: 6.9770

>AT22565 WP_012218082.1 NC_010087:c408466-408215 [Burkholderia multivorans ATCC 17616]
MELKIQKWGNSAAVRLPAVLLEQMHASVGGSLNADVRPDGVLLTPARRKYALDELVAQCDAKAPFPADMAAWGEVRPVGR
EGW

Nucleotide

• Length: 252 bp

>AT22565 NC_010087:c408466-408215 [Burkholderia multivorans ATCC 17616]
ATGGAACTAAAAATTCAGAAATGGGGCAACAGTGCGGCGGTGCGACTCCCCGCCGTGTTGCTCGAGCAGATGCACGCCTC
GGTCGGCGGCTCGCTGAACGCCGACGTGCGTCCGGACGGCGTATTGCTGACCCCCGCGCGCCGCAAGTATGCGCTGGACG
AACTGGTCGCTCAGTGTGATGCGAAAGCGCCTTTCCCGGCGGACATGGCTGCATGGGGTGAGGTCAGGCCGGTAGGGCGT
GAAGGATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A4C4A8

Antitoxin

• AlphaFold DB: A0A2A4C1X6