Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5427915..5428540 Replicon chromosome
Accession NZ_CP087606
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01304

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LMH54_RS26685 Protein ID WP_002882817.1
Coordinates 5427915..5428298 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LMH54_RS26690 Protein ID WP_004150355.1
Coordinates 5428298..5428540 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH54_RS26670 (LMH54_26670) 5425281..5426183 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LMH54_RS26675 (LMH54_26675) 5426180..5426815 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LMH54_RS26680 (LMH54_26680) 5426812..5427741 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LMH54_RS26685 (LMH54_26685) 5427915..5428298 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LMH54_RS26690 (LMH54_26690) 5428298..5428540 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LMH54_RS26695 (LMH54_26695) 5428745..5429662 + 918 WP_002882812.1 alpha/beta hydrolase -
LMH54_RS26700 (LMH54_26700) 5429676..5430617 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LMH54_RS26705 (LMH54_26705) 5430662..5431099 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LMH54_RS26710 (LMH54_26710) 5431096..5431956 - 861 WP_002882807.1 virulence factor BrkB family protein -
LMH54_RS26715 (LMH54_26715) 5431950..5432549 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T225644 WP_002882817.1 NZ_CP087606:c5428298-5427915 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T225644 NZ_CP121246:c2066881-2066579 [Agrobacterium pusense]
ATGCAGATCAAGCAATCTACTACGTTCAAAAAATGGTTTGGGAAGCTGAAGGATGAACGCGGCAAAGCTATTATTGCGTC
CCGCTTGAACAGGCTTTCCTTTGGTCACGCAGGTGACGTTGCGCCGGTTGGCAATGGAATCAGCGAATTGCGCATCCACT
ACGGGCCGGGCTATCGGGTCTATTTCATCATTTCGGATGATGTCGTTGTCGTTCTCCTTTGTGGCGGTAACAAGAGCACG
CAGGAGAAGGACATAAGAGCCGCAAGGGTGATTGCGGCGCAATGGAGTGATGACGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT225644 WP_004150355.1 NZ_CP087606:c5428540-5428298 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT225644 NZ_CP121246:c2066586-2066296 [Agrobacterium pusense]
ATGAGTGAAAAGTTCACGAACTTCAATCCTGCCGAACACCTCAAGAGCGATGAAGCAATTGAAGTATTTTTGGAGGACGC
GTTCGCAACCAACGACGCGGCATACATAGCAAATGCGCTTGGTGTTATCGCGCGCGCAAAGGGTATGACGCGGGTGGCTC
GGGAAACCGGACTTGCCAGAGAGCATCTCTACAAATCCCTCAGCGAGGCAGGAAATCCGACGTTAGAAACCACAATAGCC
GTTCTCAACGCCCTGGGCTTGCAACTCACCAGCAAACACAAGGCAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References