Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4800636..4801446 Replicon chromosome
Accession NZ_CP087606
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01304

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LMH54_RS23710 Protein ID WP_002887280.1
Coordinates 4800636..4801169 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LMH54_RS23715 Protein ID WP_002887278.1
Coordinates 4801180..4801446 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH54_RS23705 (LMH54_23705) 4799467..4800588 + 1122 WP_002887282.1 cupin domain-containing protein -
LMH54_RS23710 (LMH54_23710) 4800636..4801169 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LMH54_RS23715 (LMH54_23715) 4801180..4801446 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LMH54_RS23720 (LMH54_23720) 4801549..4802982 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
LMH54_RS23725 (LMH54_23725) 4802972..4803655 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LMH54_RS23730 (LMH54_23730) 4803827..4805212 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LMH54_RS23735 (LMH54_23735) 4805230..4805574 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T225641 WP_002887280.1 NZ_CP087606:c4801169-4800636 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T225641 NZ_CP121233:2730688-2730810 [Enterococcus faecalis]
ATGAAAGGAGTAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATCCTAGGGTTTGGTATGTTTACCATTGCTTTGAT
TGCACTGATTGTGAAATTGCTTAAAGAAGACAAAAAGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT225641 WP_002887278.1 NZ_CP087606:c4801446-4801180 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT225641 NZ_CP121233:c2731005-2730743 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCGGGCATCTTTTATTGATGGTCTTTTTAGGGGCGTTGTGCTATAATGG
TAATGAGAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTACTAACGGTGACAAAATTTTAAAGATTTTG
AAATGACCGTCTAACTAGGCAAAGTTAAACAGCGGTCATTTCTTTTTGTCTTCTTTAAGCAATTTCACAATCAGTGCAAT
CAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References