Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4188054..4188673 Replicon chromosome
Accession NZ_CP087606
Organism Klebsiella pneumoniae subsp. pneumoniae strain DD01304

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LMH54_RS20765 Protein ID WP_002892050.1
Coordinates 4188455..4188673 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LMH54_RS20760 Protein ID WP_002892066.1
Coordinates 4188054..4188428 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH54_RS20750 (LMH54_20750) 4183206..4184399 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMH54_RS20755 (LMH54_20755) 4184422..4187568 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
LMH54_RS20760 (LMH54_20760) 4188054..4188428 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LMH54_RS20765 (LMH54_20765) 4188455..4188673 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LMH54_RS20770 (LMH54_20770) 4188832..4189398 + 567 WP_002892030.1 maltose O-acetyltransferase -
LMH54_RS20775 (LMH54_20775) 4189370..4189510 - 141 WP_004147370.1 hypothetical protein -
LMH54_RS20780 (LMH54_20780) 4189531..4190001 + 471 WP_002892026.1 YlaC family protein -
LMH54_RS20785 (LMH54_20785) 4189976..4191427 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LMH54_RS20790 (LMH54_20790) 4191528..4192226 + 699 WP_002892021.1 GNAT family protein -
LMH54_RS20795 (LMH54_20795) 4192223..4192363 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LMH54_RS20800 (LMH54_20800) 4192363..4192626 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225640 WP_002892050.1 NZ_CP087606:4188455-4188673 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225640 NZ_CP121233:c2724277-2723936 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACGCGATATACGTTGCCCGATGACATAGAAACTCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225640 WP_002892066.1 NZ_CP087606:4188054-4188428 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225640 NZ_CP121233:c2724507-2724277 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References