Detailed information of TA system TA225613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197742..2198271
• Replicon: chromosome
• Accession: NZ_CP087593
• Organism: Staphylococcus aureus strain Newman NM-CQ

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LO764_RS11205
• Protein ID: WP_000621175.1
• Coordinates: 2197742..2198104 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: LO764_RS11210
• Protein ID: WP_000948331.1
• Coordinates: 2198101..2198271 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO764_RS11185 (2194719)	2194719..2195489	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
LO764_RS11190 (2195464)	2195464..2195943	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
LO764_RS11195 (2195945)	2195945..2196271	-	327	WP_001052491.1	anti-sigma factor antagonist	-
LO764_RS11200 (2196391)	2196391..2197392	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
LO764_RS11205 (2197742)	2197742..2198104	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LO764_RS11210 (2198101)	2198101..2198271	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
LO764_RS11215 (2198356)	2198356..2199504	-	1149	WP_001281145.1	alanine racemase	-
LO764_RS11220 (2199570)	2199570..2199929	-	360	WP_000581200.1	holo-ACP synthase	-
LO764_RS11225 (2199933)	2199933..2200424	-	492	WP_001205910.1	PH domain-containing protein	-
LO764_RS11230 (2200411)	2200411..2201994	-	1584	WP_001294631.1	PH domain-containing protein	-
LO764_RS11235 (2201987)	2201987..2202466	-	480	WP_001287088.1	hypothetical protein	-
LO764_RS11240 (2202675)	2202675..2203235	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T225613 WP_000621175.1 NZ_CP087593:c2198104-2197742 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T225613 NZ_CP121222:c17833-17558 [Shigella flexneri 2a]
ATGGAACTGAAGTGGACCAGTAAGGCACTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCGCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTACTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAGAACAGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT225613 WP_000948331.1 NZ_CP087593:c2198271-2198101 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT225613 NZ_CP121222:c18117-17833 [Shigella flexneri 2a]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGTCCTGG
GCGGACAGCCTCAGTACTGACCATCTGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1