Detailed information of TA system TA225592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 25777..26420
• Replicon: plasmid pCMP234-1
• Accession: NZ_CP087581
• Organism: Klebsiella quasipneumoniae strain OSUCMP263AKPC

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LO541_RS24955
• Protein ID: WP_016236302.1
• Coordinates: 26004..26420 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: LO541_RS24950
• Protein ID: WP_001261282.1
• Coordinates: 25777..26007 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO541_RS24925 (LO541_24925)	21493..21750	-	258	WP_023292103.1	hypothetical protein	-
LO541_RS24930 (LO541_24930)	22228..22908	+	681	Protein_22	CSS-motif domain-containing protein	-
LO541_RS24935 (LO541_24935)	22972..23808	+	837	WP_032439674.1	EAL domain-containing protein	-
LO541_RS24940 (LO541_24940)	24426..24857	-	432	WP_174805835.1	hypothetical protein	-
LO541_RS24945 (LO541_24945)	25398..25820	-	423	WP_046624301.1	hypothetical protein	-
LO541_RS24950 (LO541_24950)	25777..26007	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LO541_RS24955 (LO541_24955)	26004..26420	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
LO541_RS24960 (LO541_24960)	26494..28056	+	1563	WP_004206609.1	AAA family ATPase	-
LO541_RS24965 (LO541_24965)	28041..29063	+	1023	WP_032439672.1	helicase UvrD	-
LO541_RS24970 (LO541_24970)	29608..30516	+	909	WP_032439686.1	HNH endonuclease	-
LO541_RS24975 (LO541_24975)	30702..31052	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / dfrA14	-	1..170849	170849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T225592 WP_016236302.1 NZ_CP087581:26004-26420 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T225592 NZ_CP121199:c4286361-4286011 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT225592 WP_001261282.1 NZ_CP087581:25777-26007 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT225592 NZ_CP121199:c4286606-4286355 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3