Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3932626..3933245 Replicon chromosome
Accession NZ_CP087580
Organism Klebsiella quasipneumoniae strain OSUCMP263AKPC

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LO541_RS19125 Protein ID WP_002892050.1
Coordinates 3933027..3933245 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LO541_RS19120 Protein ID WP_002892066.1
Coordinates 3932626..3933000 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO541_RS19110 (3927779) 3927779..3928972 + 1194 WP_004204747.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LO541_RS19115 (3928995) 3928995..3932141 + 3147 WP_004204748.1 multidrug efflux RND transporter permease subunit AcrB -
LO541_RS19120 (3932626) 3932626..3933000 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LO541_RS19125 (3933027) 3933027..3933245 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LO541_RS19130 (3933404) 3933404..3933970 + 567 WP_004204750.1 maltose O-acetyltransferase -
LO541_RS19135 (3933942) 3933942..3934064 - 123 WP_032431198.1 hypothetical protein -
LO541_RS19140 (3934107) 3934107..3934577 + 471 WP_060656956.1 YlaC family protein -
LO541_RS19145 (3934546) 3934546..3936003 - 1458 WP_060656955.1 PLP-dependent aminotransferase family protein -
LO541_RS19150 (3936104) 3936104..3936802 + 699 WP_169319236.1 GNAT family protein -
LO541_RS19155 (3936799) 3936799..3936939 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LO541_RS19160 (3936939) 3936939..3937202 - 264 WP_169319235.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225591 WP_002892050.1 NZ_CP087580:3933027-3933245 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T225591 NZ_CP121199:c4274957-4274667 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT225591 WP_002892066.1 NZ_CP087580:3932626-3933000 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT225591 NZ_CP121199:c4275189-4274947 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References