Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5632..6157 Replicon plasmid pCMP42-1
Accession NZ_CP087579
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KUA31_RS23820 Protein ID WP_001159871.1
Coordinates 5852..6157 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KUA31_RS23815 Protein ID WP_000813630.1
Coordinates 5632..5850 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS23790 (932) 932..1528 + 597 Protein_2 IS1 family transposase -
KUA31_RS23795 (1782) 1782..2804 - 1023 WP_000361402.1 helicase UvrD -
KUA31_RS23800 (2789) 2789..4354 - 1566 WP_001128474.1 AAA family ATPase -
KUA31_RS23805 (4429) 4429..4845 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KUA31_RS23810 (4842) 4842..5072 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KUA31_RS23815 (5632) 5632..5850 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KUA31_RS23820 (5852) 5852..6157 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KUA31_RS23825 (6158) 6158..6964 + 807 WP_000016970.1 site-specific integrase -
KUA31_RS23830 (7686) 7686..8441 + 756 WP_000852146.1 replication initiation protein RepE -
KUA31_RS23835 (9029) 9029..10195 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) - 1..120124 120124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T225579 WP_001159871.1 NZ_CP087579:5852-6157 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T225579 NZ_CP121199:1106801-1107136 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTCAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTTGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT225579 WP_000813630.1 NZ_CP087579:5632-5850 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT225579 NZ_CP121199:1106553-1106801 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGGCCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAGCGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References