Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4429..5072 Replicon plasmid pCMP42-1
Accession NZ_CP087579
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KUA31_RS23805 Protein ID WP_001034044.1
Coordinates 4429..4845 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KUA31_RS23810 Protein ID WP_001261286.1
Coordinates 4842..5072 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS23785 (219) 219..916 + 698 WP_223350503.1 IS1 family transposase -
KUA31_RS23790 (932) 932..1528 + 597 Protein_2 IS1 family transposase -
KUA31_RS23795 (1782) 1782..2804 - 1023 WP_000361402.1 helicase UvrD -
KUA31_RS23800 (2789) 2789..4354 - 1566 WP_001128474.1 AAA family ATPase -
KUA31_RS23805 (4429) 4429..4845 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KUA31_RS23810 (4842) 4842..5072 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KUA31_RS23815 (5632) 5632..5850 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KUA31_RS23820 (5852) 5852..6157 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KUA31_RS23825 (6158) 6158..6964 + 807 WP_000016970.1 site-specific integrase -
KUA31_RS23830 (7686) 7686..8441 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) - 1..120124 120124


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T225578 WP_001034044.1 NZ_CP087579:c4845-4429 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T225578 NZ_CP121199:1005153-1005560 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT225578 WP_001261286.1 NZ_CP087579:c5072-4842 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT225578 NZ_CP121199:1004906-1005172 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References