Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3923286..3923940 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KUA31_RS19275 Protein ID WP_000244777.1
Coordinates 3923286..3923693 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KUA31_RS19280 Protein ID WP_000354046.1
Coordinates 3923674..3923940 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS19255 (3919243) 3919243..3920976 - 1734 WP_230282971.1 single-stranded-DNA-specific exonuclease RecJ -
KUA31_RS19260 (3920982) 3920982..3921692 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KUA31_RS19265 (3921717) 3921717..3922613 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KUA31_RS19270 (3922725) 3922725..3923246 + 522 WP_001055874.1 flavodoxin FldB -
KUA31_RS19275 (3923286) 3923286..3923693 - 408 WP_000244777.1 protein YgfX Toxin
KUA31_RS19280 (3923674) 3923674..3923940 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KUA31_RS19285 (3924183) 3924183..3925163 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KUA31_RS19290 (3925416) 3925416..3926396 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
KUA31_RS19295 (3926519) 3926519..3927178 - 660 WP_000250274.1 hemolysin III family protein -
KUA31_RS19300 (3927342) 3927342..3927653 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3925416..3926396 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T225575 WP_000244777.1 NZ_CP087578:c3923693-3923286 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T225575 NZ_CP121199:c654090-653626 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT225575 WP_000354046.1 NZ_CP087578:c3923940-3923674 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225575 NZ_CP121199:c654425-654090 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References