Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3500347..3500972 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag KUA31_RS17255 Protein ID WP_000911328.1
Coordinates 3500347..3500745 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KUA31_RS17260 Protein ID WP_000450524.1
Coordinates 3500745..3500972 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS17235 (3496225) 3496225..3496425 + 201 WP_000383836.1 YpfN family protein -
KUA31_RS17240 (3496535) 3496535..3497233 - 699 WP_000679812.1 esterase -
KUA31_RS17245 (3497307) 3497307..3499322 - 2016 WP_075826571.1 tRNA cytosine(34) acetyltransferase TmcA -
KUA31_RS17250 (3499337) 3499337..3500200 - 864 WP_001267495.1 neutral zinc metallopeptidase -
KUA31_RS17255 (3500347) 3500347..3500745 - 399 WP_000911328.1 tRNA(fMet)-specific endonuclease VapC Toxin
KUA31_RS17260 (3500745) 3500745..3500972 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KUA31_RS17265 (3501126) 3501126..3501839 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KUA31_RS17270 (3502052) 3502052..3503086 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KUA31_RS17275 (3503103) 3503103..3503981 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KUA31_RS17280 (3504127) 3504127..3504699 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KUA31_RS17285 (3504699) 3504699..3505169 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14966.32 Da        Isoelectric Point: 8.5325

>T225572 WP_000911328.1 NZ_CP087578:c3500745-3500347 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYEAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T225572 NZ_CP121199:240676-240783 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT225572 WP_000450524.1 NZ_CP087578:c3500972-3500745 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT225572 NZ_CP121199:c240627-240564 [Escherichia coli]
GTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References