Detailed information of TA system TA225570

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2965744..2966579
• Replicon: chromosome
• Accession: NZ_CP087578
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KUA31_RS14725
• Protein ID: WP_001461142.1
• Coordinates: 2966202..2966579 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KUA31_RS14720
• Protein ID: WP_001295723.1
• Coordinates: 2965744..2966112 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS14690 (2961783)	2961783..2962529	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KUA31_RS14695 (2962853)	2962853..2963048	+	196	Protein_2783	DUF905 family protein	-
KUA31_RS14700 (2963166)	2963166..2963984	+	819	WP_001234729.1	DUF932 domain-containing protein	-
KUA31_RS14705 (2964326)	2964326..2964799	+	474	WP_000855059.1	antirestriction protein	-
KUA31_RS14710 (2964815)	2964815..2965291	+	477	WP_001186770.1	RadC family protein	-
KUA31_RS14715 (2965360)	2965360..2965581	+	222	WP_000692353.1	DUF987 domain-containing protein	-
KUA31_RS14720 (2965744)	2965744..2966112	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KUA31_RS14725 (2966202)	2966202..2966579	+	378	WP_001461142.1	TA system toxin CbtA family protein	Toxin
KUA31_RS14730 (2966576)	2966576..2966725	+	150	Protein_2790	DUF5983 family protein	-
KUA31_RS14735 (2966825)	2966825..2966905	+	81	Protein_2791	hypothetical protein	-
KUA31_RS14740 (2967721)	2967721..2968092	+	372	WP_001295631.1	IS110 family transposase	-
KUA31_RS14745 (2968363)	2968363..2968728	+	366	WP_226234444.1	EutP/PduV family microcompartment system protein	-
KUA31_RS14750 (2968829)	2968829..2969158	-	330	WP_000450409.1	DUF496 family protein	-
KUA31_RS14755 (2969330)	2969330..2970388	-	1059	WP_001200892.1	FUSC family protein	-
KUA31_RS14760 (2970586)	2970586..2971059	-	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14183.21 Da
• Isoelectric Point: 7.3249

>T225570 WP_001461142.1 NZ_CP087578:2966202-2966579 [Escherichia coli]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T225570 NZ_CP121193:3482107-3482373 [Halomonas sp. CKK8]
ATGGATGCCTTCGAGTTCGACTCGGGCAAGAGCGAAGCTAACAGGCACAAGCACGGCATCGACTTTGTCGACGCCCAGCG
CCTCTGGGAAGACCCGGACTTGCTTGAGATCCCGGCAAAGACCATGGATGAGCCACGTTTCGTCGTGATTGGCAGGATCG
ATGGCAAGCACTGGTCCGGCATCATCACGTACCGCGGTAGACGCATTCGCCTGATCTCGGTCAGACGTTCGCGCAGCAGT
GAGGTCGCCCTCTATGAAAGCCCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT225570 WP_001295723.1 NZ_CP087578:2965744-2966112 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT225570 NZ_CP121193:3482360-3482596 [Halomonas sp. CKK8]
ATGAAAGCCCATGATCTCGATAACCGCTTCGATGACGGTGACGACATCATCGATGAGCTCGACTTGTCGCAAGCCAGTCG
TCCGCTCAGAAAGCAGAAGCGCGTCAACGTCGATTTCCCGACCTGGATGATCGACTCGCTGGACCGGGAGGCCGCGCGTC
TCGGCGTCACCCGTCAGTCCATCATCAAGATCTGGCTGGCCGAACGCCTGGAGCAGCACGGCTCGCATTCGCACTGA

Structures

Antitoxin

• AlphaFold DB: S1NM52