Detailed information of TA system TA225561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2450707..2452295
• Replicon: chromosome
• Accession: NZ_CP087578
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KUA31_RS12070
• Protein ID: WP_125291386.1
• Coordinates: 2450707..2452029 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KUA31_RS12075
• Protein ID: WP_001296726.1
• Coordinates: 2452029..2452295 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS12050 (2446384)	2446384..2446947	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
KUA31_RS12055 (2447379)	2447379..2447587	-	209	Protein_2267	AraC family transcriptional regulator	-
KUA31_RS12060 (2447941)	2447941..2449338	+	1398	WP_075861455.1	ISNCY family transposase	-
KUA31_RS12065 (2449573)	2449573..2450514	+	942	WP_000974761.1	hypothetical protein	-
KUA31_RS12070 (2450707)	2450707..2452029	-	1323	WP_125291386.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KUA31_RS12075 (2452029)	2452029..2452295	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2433833..2452476	18643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49407.04 Da
• Isoelectric Point: 9.1161

>T225561 WP_125291386.1 NZ_CP087578:c2452029-2450707 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGSVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLRREYGSK

Nucleotide

• Length: 1323 bp

>T225561 NZ_CP121189:c4329501-4329217 [Salmonella enterica subsp. enterica serovar Indiana]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT225561 WP_001296726.1 NZ_CP087578:c2452295-2452029 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT225561 NZ_CP121189:c4329733-4329491 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTACGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA