Detailed information of TA system TA225560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2356564..2357202
• Replicon: chromosome
• Accession: NZ_CP087578
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KUA31_RS11660
• Protein ID: WP_000813794.1
• Coordinates: 2356564..2356740 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KUA31_RS11665
• Protein ID: WP_001270286.1
• Coordinates: 2356786..2357202 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS11640 (2352183)	2352183..2353358	-	1176	WP_230283012.1	BenE family transporter YdcO	-
KUA31_RS11645 (2353450)	2353450..2353986	+	537	WP_000429152.1	DNA-binding transcriptional regulator SutR	-
KUA31_RS11650 (2354059)	2354059..2356020	+	1962	WP_075826559.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KUA31_RS11655 (2356112)	2356112..2356342	-	231	WP_000494244.1	YncJ family protein	-
KUA31_RS11660 (2356564)	2356564..2356740	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KUA31_RS11665 (2356786)	2356786..2357202	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KUA31_RS11670 (2357281)	2357281..2358687	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
KUA31_RS11675 (2358932)	2358932..2360077	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KUA31_RS11680 (2360095)	2360095..2361108	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KUA31_RS11685 (2361109)	2361109..2362050	+	942	WP_075826558.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2350995..2352143	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T225560 WP_000813794.1 NZ_CP087578:2356564-2356740 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T225560 NZ_CP121189:3831698-3831949 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCGGGATACTTACAAAACCCACATGACCACCTATTACTCGCGCATCCCCAGCCTGCACCTTAAGGGCGACTGGCTGGC
AGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGATCTCCGAAGGGTGTTTGACTCTGATTGTGGAGACAGACG
AAGTGAGGGATCTACGGAGGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCACATTAAGGCGGGGGTATATGATGTGGTG
AACGGGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT225560 WP_001270286.1 NZ_CP087578:2356786-2357202 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT225560 NZ_CP121189:c3831939-3831714 [Salmonella enterica subsp. enterica serovar Indiana]
TTCACCACATCATATACCCCCGCCTTAATGTGCTTCATCGACTTTTTCACCTGATAAAGCTCCCTCCGTAGATCCCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCTTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCAGGCTGGGGATGCGCGAGTAATAGGTGGTCATGTGGGTTT

Structures

Toxin

• PDB: 6HPB