Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1325661..1326279 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KUA31_RS06675 Protein ID WP_001291435.1
Coordinates 1325661..1325879 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KUA31_RS06680 Protein ID WP_000344800.1
Coordinates 1325905..1326279 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS06640 (1320950) 1320950..1321522 + 573 WP_000779831.1 YbaY family lipoprotein -
KUA31_RS06645 (1321553) 1321553..1321864 - 312 WP_000409911.1 MGMT family protein -
KUA31_RS06655 (1322243) 1322243..1322596 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KUA31_RS06660 (1322638) 1322638..1324188 - 1551 WP_075826856.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KUA31_RS06665 (1324352) 1324352..1324822 - 471 WP_000136192.1 YlaC family protein -
KUA31_RS06670 (1324938) 1324938..1325489 - 552 WP_075826849.1 maltose O-acetyltransferase -
KUA31_RS06675 (1325661) 1325661..1325879 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KUA31_RS06680 (1325905) 1325905..1326279 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KUA31_RS06685 (1326825) 1326825..1329974 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KUA31_RS06690 (1329997) 1329997..1331190 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225553 WP_001291435.1 NZ_CP087578:c1325879-1325661 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T225553 NZ_CP121189:933090-933503 [Salmonella enterica subsp. enterica serovar Indiana]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCATCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT225553 WP_000344800.1 NZ_CP087578:c1326279-1325905 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT225553 NZ_CP121189:932843-933109 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References