Detailed information of TA system TA225552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1291363..1292200
• Replicon: chromosome
• Accession: NZ_CP087578
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KUA31_RS06505
• Protein ID: WP_000227784.1
• Coordinates: 1291363..1291905 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KUA31_RS06510
• Protein ID: WP_001297137.1
• Coordinates: 1291889..1292200 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS06485 (1286902)	1286902..1287813	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
KUA31_RS06490 (1287981)	1287981..1288472	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
KUA31_RS06495 (1288600)	1288600..1289964	-	1365	WP_001000974.1	MFS transporter	-
KUA31_RS06500 (1290372)	1290372..1291307	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
KUA31_RS06505 (1291363)	1291363..1291905	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KUA31_RS06510 (1291889)	1291889..1292200	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KUA31_RS06515 (1292385)	1292385..1293275	-	891	WP_000971336.1	heme o synthase	-
KUA31_RS06520 (1293287)	1293287..1293616	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KUA31_RS06525 (1293616)	1293616..1294230	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KUA31_RS06530 (1294220)	1294220..1296211	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KUA31_RS06535 (1296233)	1296233..1297180	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T225552 WP_000227784.1 NZ_CP087578:c1291905-1291363 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T225552 NZ_CP121189:692964-693347 [Salmonella enterica subsp. enterica serovar Indiana]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT225552 WP_001297137.1 NZ_CP087578:c1292200-1291889 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT225552 NZ_CP121189:692506-692874 [Salmonella enterica subsp. enterica serovar Indiana]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAATCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3