Detailed information of TA system TA225550

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1108577..1109412
• Replicon: chromosome
• Accession: NZ_CP087578
• Organism: Escherichia coli strain OSUCMP42NDM

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: KUA31_RS05625
• Protein ID: WP_064995992.1
• Coordinates: 1109035..1109412 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KUA31_RS05620
• Protein ID: WP_064995991.1
• Coordinates: 1108577..1108945 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA31_RS05590 (1103744)	1103744..1104562	+	819	WP_064995988.1	DUF932 domain-containing protein	-
KUA31_RS05595 (1104805)	1104805..1106346	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
KUA31_RS05600 (1106361)	1106361..1107107	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
KUA31_RS05605 (1107240)	1107240..1107725	+	486	WP_064995989.1	antirestriction protein	-
KUA31_RS05610 (1107741)	1107741..1108217	+	477	WP_064995990.1	RadC family protein	-
KUA31_RS05615 (1108280)	1108280..1108501	+	222	WP_000692323.1	DUF987 domain-containing protein	-
KUA31_RS05620 (1108577)	1108577..1108945	+	369	WP_064995991.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KUA31_RS05625 (1109035)	1109035..1109412	+	378	WP_064995992.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KUA31_RS05630 (1109409)	1109409..1109606	+	198	Protein_1006	DUF5983 family protein	-
KUA31_RS05635 (1109634)	1109634..1109831	+	198	WP_085949090.1	DUF957 domain-containing protein	-
KUA31_RS05640 (1109916)	1109916..1110764	+	849	WP_001280470.1	DUF4942 domain-containing protein	-
KUA31_RS05645 (1110857)	1110857..1112176	-	1320	WP_000144688.1	site-specific integrase	-
KUA31_RS05650 (1112478)	1112478..1113689	+	1212	WP_001460479.1	tyrosine-type recombinase/integrase	-
KUA31_RS05655 (1113903)	1113903..1114058	-	156	WP_230283015.1	DUF4431 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1089414..1116536	27122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.00 Da
• Isoelectric Point: 8.5163

>T225550 WP_064995992.1 NZ_CP087578:1109035-1109412 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDIYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T225550 NZ_CP121160:1045582-1045917 [Streptococcus agalactiae]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCCCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAATGACATAGAAAAACTTGAAGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAGAAATCAGCAATTATCACAGTACTAGAGGTTACACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAAGAGGAAGAGAATAGGGTTGTCATTGATTACTTGCTTCCTACTCGAAGCGACTATAT
GAAATTGTTTAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13579.32 Da
• Isoelectric Point: 5.5269

>AT225550 WP_064995991.1 NZ_CP087578:1108577-1108945 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETTK

Nucleotide

• Length: 369 bp

>AT225550 NZ_CP121160:1045305-1045592 [Streptococcus agalactiae]
ATGGTTACAGTAGAAAAAAATAGAGCTGTCACATTCCAAGCTAACAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCCGAAATCAGCAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA