Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 450061..450558 Replicon chromosome
Accession NZ_CP087578
Organism Escherichia coli strain OSUCMP42NDM

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KUA31_RS02535 Protein ID WP_001173343.1
Coordinates 450385..450558 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KUA31_RS02530 Protein ID WP_000398619.1
Coordinates 450061..450357 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUA31_RS02510 (446792) 446792..447511 - 720 WP_000611288.1 two-component system response regulator DcuR -
KUA31_RS02515 (447508) 447508..449139 - 1632 WP_001216487.1 sensor histidine kinase -
KUA31_RS02520 (449320) 449320..449550 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KUA31_RS02525 (449562) 449562..449834 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
KUA31_RS02530 (450061) 450061..450357 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KUA31_RS02535 (450385) 450385..450558 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KUA31_RS02540 (450677) 450677..452194 - 1518 WP_001295074.1 lysine--tRNA ligase -
KUA31_RS02545 (452431) 452431..453888 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T225546 WP_001173343.1 NZ_CP087578:450385-450558 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T225546 NZ_CP121159:c1088274-1087429 [Streptococcus agalactiae]
ATGAATAAAGCTAAGCTAACAGCACTCTGTCATAAAATATCTAAGAATACTGGATTAACCTTTAATTCCGTGATGACCTA
TTACTTTCTAGAAGTGATTTTGAAAAAATTAAGTCAGAGTACCTACTCAAATCACTATATCTTCAAAGGAGGATTCCTAC
TGTCAAATGTTATTGGAGTTGAATCTCGTAGCACGGTTGATATTGATTTTTTGTTCCATCAAATAACACTCTCAGAAGAT
ACTGTGAAGCAGCAACTCAAAGAAATTTTAGCAGATTCAGAAGAAGGTATATCTTTTGTGATTCAATCCATCACAGCTAT
TAAAGAAAGTGATGACTATGGTGGCTATCGAGCAACAATTTTGTGCCAACTTGAGAATATTAAACAAGTTATCCATTTGG
ATATTGCGACAGGCGATGTTGTAACTCCTCAGCCGATTACATACGACTATAAAGCTATTTTTGATGAAGAAAATTTTCCA
ATCATTGCTTATACAATTGAAACAATCCTAGCTGAGAAACTTCAAACCATCTATTCACGTAACTTCTTGAACAGCAGAAG
CAAAGATTTTTACGATGTTTATATTCTTTCAAAACTGAAGAAAGAAGACATTGACTTCATTCAATTGAAAAATGCCTGTC
AGAGAACATTTTCATATCGCGAAACAGTGCTCGATTTTGAAAAGATTATTGAACTACTCGAGAGGTTCAAATCTGACCCT
ATTCAGAATCAACAATGGCAAAATTATTCGAAAAAATATAGCTATACAAAGGGAATTTCCCTTGCAGATGTTCTTGAGGA
GATGATTCGTCTCATAGCAGTTCTCATTTCTATAAATTTTGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT225546 WP_000398619.1 NZ_CP087578:450061-450357 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT225546 NZ_CP121159:c1088861-1088271 [Streptococcus agalactiae]
ATGTCAAAAAAAGAGATTCTACTTGAATTTATAGAAAACCACAATGGCATTATCACCTATAAAGATTGTAAAGCTCTAAA
TATTCCAACAATATATTTGACAAGACTAGAAAAAGAAGGAATTCTATACCGCGTCGAAAAAGGAATTTTCCTAACCCAGA
ATGGAGACTACGACGAATACTACTTCTTTCAGTACCGATATCCGAAAGCTGTTTTCTCATATATCTCAGCGCTTTATCTG
CAACAATTTACAGATGAAATTCCTCAATATTTTGACGTAACCATTCCTAGAGGTTACCGTTTTAATACTCCTCCAGCTAA
TCTGAACATTCATTCCGTTTCTAAGGAAAATAGCGAACTAGGAATTACTCTTGTAAAAACCCCTATGGGAAACGAGGTAA
GAGTGTATGATTTCGAACGTATTATCTGTGATTTTGTGATTCATCGAGAAAAAATAGATACTGAGCTTTTTGTCAAAACA
CTTCAACTTTACGGAAACTATCCTAAAAAAAATCTTACAAAACTCTATGAATATGCGACAAAAATGAGCACCTTGGCCAA
GGTCAAACAAACTCTGGAGGTCCTAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References