225537

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 63676..64277 Replicon plasmid p1OXA77
Accession NZ_CP087571
Organism Escherichia coli strain 114

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LOS76_RS23335 Protein ID WP_001216034.1
Coordinates 63897..64277 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LOS76_RS23330 Protein ID WP_001190712.1
Coordinates 63676..63897 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOS76_RS23320 (60657) 60657..61940 - 1284 WP_001617890.1 restriction endonuclease subunit S -
LOS76_RS23325 (61937) 61937..63493 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
LOS76_RS23330 (63676) 63676..63897 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LOS76_RS23335 (63897) 63897..64277 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LOS76_RS23340 (64282) 64282..64461 + 180 WP_001513661.1 hypothetical protein -
LOS76_RS23345 (64489) 64489..64767 + 279 Protein_70 pdcB -
LOS76_RS23350 (64772) 64772..65185 + 414 Protein_71 integrase core domain-containing protein -
LOS76_RS23355 (65981) 65981..66958 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
LOS76_RS23360 (67243) 67243..67983 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
LOS76_RS23365 (68104) 68104..68286 - 183 WP_042065278.1 hypothetical protein -
LOS76_RS23370 (68353) 68353..68642 - 290 Protein_75 type II toxin-antitoxin system RelE/ParE family toxin -
LOS76_RS23375 (68629) 68629..68897 - 269 Protein_76 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / mph(A) / aac(3)-IId / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / fosA3 / blaTEM-1B / blaCTX-M-55 - 1..151915 151915
- flank IS/Tn - - 64811..65185 374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T225537 WP_001216034.1 NZ_CP087571:63897-64277 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T225537 NZ_CP121157:c2659923-2659820 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT225537 WP_001190712.1 NZ_CP087571:63676-63897 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT225537 NZ_CP121157:2659782-2660053 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGTTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References