Detailed information of TA system TA225519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2720981..2721606
• Replicon: chromosome
• Accession: NZ_CP087570
• Organism: Escherichia coli strain 114

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LOS76_RS13065
• Protein ID: WP_000911330.1
• Coordinates: 2721208..2721606 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LOS76_RS13060
• Protein ID: WP_000450524.1
• Coordinates: 2720981..2721208 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOS76_RS13035 (2716784)	2716784..2717254	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LOS76_RS13040 (2717254)	2717254..2717826	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LOS76_RS13045 (2717972)	2717972..2718850	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LOS76_RS13050 (2718867)	2718867..2719901	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LOS76_RS13055 (2720114)	2720114..2720827	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LOS76_RS13060 (2720981)	2720981..2721208	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LOS76_RS13065 (2721208)	2721208..2721606	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LOS76_RS13070 (2721753)	2721753..2722616	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
LOS76_RS13075 (2722631)	2722631..2724646	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
LOS76_RS13080 (2724720)	2724720..2725061	+	342	Protein_2551	esterase	-
LOS76_RS13085 (2725143)	2725143..2725889	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T225519 WP_000911330.1 NZ_CP087570:2721208-2721606 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T225519 NZ_CP121155:3678620-3679162 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT225519 WP_000450524.1 NZ_CP087570:2720981-2721208 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT225519 NZ_CP121155:3678325-3678636 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33