Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2280007..2280661 Replicon chromosome
Accession NZ_CP087570
Organism Escherichia coli strain 114

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag LOS76_RS10970 Protein ID WP_000244777.1
Coordinates 2280254..2280661 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LOS76_RS10965 Protein ID WP_000354046.1
Coordinates 2280007..2280273 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOS76_RS10940 (2275295) 2275295..2276038 + 744 Protein_2135 SDR family oxidoreductase -
LOS76_RS10945 (2276095) 2276095..2277528 - 1434 WP_138412971.1 6-phospho-beta-glucosidase BglA -
LOS76_RS10950 (2277573) 2277573..2277884 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
LOS76_RS10955 (2278048) 2278048..2278707 + 660 WP_000250274.1 hemolysin III family protein -
LOS76_RS10960 (2278784) 2278784..2279764 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LOS76_RS10965 (2280007) 2280007..2280273 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LOS76_RS10970 (2280254) 2280254..2280661 + 408 WP_000244777.1 protein YgfX Toxin
LOS76_RS10975 (2280701) 2280701..2281222 - 522 WP_001055874.1 flavodoxin FldB -
LOS76_RS10980 (2281334) 2281334..2282230 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LOS76_RS10985 (2282255) 2282255..2282965 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LOS76_RS10990 (2282971) 2282971..2284704 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T225516 WP_000244777.1 NZ_CP087570:2280254-2280661 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T225516 NZ_CP121155:3217814-3218200 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT225516 WP_000354046.1 NZ_CP087570:2280007-2280273 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT225516 NZ_CP121155:3218190-3218519 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References