Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1716693..1717493 Replicon chromosome
Accession NZ_CP087570
Organism Escherichia coli strain 114

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag LOS76_RS08135 Protein ID WP_000342449.1
Coordinates 1716966..1717493 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LOS76_RS08130 Protein ID WP_001277108.1
Coordinates 1716693..1716959 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOS76_RS08110 (1712350) 1712350..1713018 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LOS76_RS08115 (1713011) 1713011..1714069 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
LOS76_RS08120 (1714314) 1714314..1715168 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
LOS76_RS08125 (1715440) 1715440..1716543 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LOS76_RS08130 (1716693) 1716693..1716959 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LOS76_RS08135 (1716966) 1716966..1717493 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LOS76_RS08140 (1717490) 1717490..1717873 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
LOS76_RS08145 (1718297) 1718297..1719406 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LOS76_RS08150 (1719454) 1719454..1720380 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LOS76_RS08155 (1720377) 1720377..1721654 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
LOS76_RS08160 (1721651) 1721651..1722418 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T225513 WP_000342449.1 NZ_CP087570:1716966-1717493 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T225513 NZ_CP121155:c2155079-2154972 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT225513 WP_001277108.1 NZ_CP087570:1716693-1716959 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT225513 NZ_CP121155:2155126-2155192 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References