Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1695402..1695988 Replicon chromosome
Accession NZ_CP087570
Organism Escherichia coli strain 114

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LOS76_RS08010 Protein ID WP_042014162.1
Coordinates 1695402..1695632 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LOS76_RS08015 Protein ID WP_000593555.1
Coordinates 1695629..1695988 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOS76_RS08000 (1691439) 1691439..1694174 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
LOS76_RS08005 (1694174) 1694174..1695298 + 1125 WP_001314210.1 ABC transporter permease -
LOS76_RS08010 (1695402) 1695402..1695632 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LOS76_RS08015 (1695629) 1695629..1695988 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LOS76_RS08020 (1696108) 1696108..1696509 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LOS76_RS08025 (1696515) 1696515..1697321 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LOS76_RS08030 (1697318) 1697318..1698082 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LOS76_RS08035 (1698082) 1698082..1698915 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LOS76_RS08040 (1698912) 1698912..1699856 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T225512 WP_042014162.1 NZ_CP087570:1695402-1695632 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T225512 NZ_CP121155:c2154543-2154436 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT225512 WP_000593555.1 NZ_CP087570:1695629-1695988 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT225512 NZ_CP121155:2154596-2154657 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References