Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 74297..74898 | Replicon | plasmid p10_578_3 |
| Accession | NZ_CP087567 | ||
| Organism | Escherichia coli strain CM2010-0578/1 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | U9YA20 |
| Locus tag | LOF66_RS26540 | Protein ID | WP_001216045.1 |
| Coordinates | 74297..74677 (-) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | LOF66_RS26545 | Protein ID | WP_001190712.1 |
| Coordinates | 74677..74898 (-) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOF66_RS26515 (LOF66_26385) | 69737..71221 | - | 1485 | WP_087762050.1 | terminase | - |
| LOF66_RS26520 (LOF66_26390) | 71221..72414 | - | 1194 | WP_050153510.1 | hypothetical protein | - |
| LOF66_RS26525 (LOF66_26395) | 72501..72953 | - | 453 | WP_001312282.1 | late promoter-activating protein (Gp10) | - |
| LOF66_RS26530 (LOF66_26400) | 73042..74085 | - | 1044 | WP_050153511.1 | DUF968 domain-containing protein | - |
| LOF66_RS26535 (LOF66_26405) | 74113..74292 | - | 180 | WP_000113019.1 | hypothetical protein | - |
| LOF66_RS26540 (LOF66_26410) | 74297..74677 | - | 381 | WP_001216045.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| LOF66_RS26545 (LOF66_26415) | 74677..74898 | - | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| LOF66_RS26550 (LOF66_26420) | 74971..75360 | - | 390 | WP_000506726.1 | S24 family peptidase | - |
| LOF66_RS26555 (LOF66_26425) | 75484..75735 | - | 252 | WP_001377386.1 | DNA polymerase III subunit theta | - |
| LOF66_RS26560 (LOF66_26430) | 76185..76322 | + | 138 | WP_000123562.1 | hypothetical protein | - |
| LOF66_RS26565 (LOF66_26435) | 76397..76759 | - | 363 | WP_094307767.1 | hypothetical protein | - |
| LOF66_RS26570 (LOF66_26440) | 76756..77688 | - | 933 | WP_000057449.1 | hypothetical protein | - |
| LOF66_RS26575 (LOF66_26445) | 77670..78044 | - | 375 | WP_000988658.1 | hypothetical protein | - |
| LOF66_RS26580 (LOF66_26450) | 78051..78344 | - | 294 | WP_050153512.1 | hypothetical protein | - |
| LOF66_RS26585 (LOF66_26455) | 78523..78756 | - | 234 | WP_000516537.1 | hypothetical protein | - |
| LOF66_RS26590 (LOF66_26460) | 78839..79252 | - | 414 | Protein_87 | DUF551 domain-containing protein | - |
| LOF66_RS26595 (LOF66_26465) | 79424..79603 | - | 180 | Protein_88 | hypothetical protein | - |
| LOF66_RS26600 (LOF66_26470) | 79619..79747 | - | 129 | Protein_89 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..99008 | 99008 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13588.29 Da Isoelectric Point: 5.1514
>T225508 WP_001216045.1 NZ_CP087567:c74677-74297 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T225508 NZ_CP121155:1005153-1005560 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT225508 WP_001190712.1 NZ_CP087567:c74898-74677 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT225508 NZ_CP121155:1004906-1005172 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CLP7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |