Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23069..23712 Replicon plasmid p10_578_2
Accession NZ_CP087566
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag LOF66_RS25730 Protein ID WP_001034046.1
Coordinates 23069..23485 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag LOF66_RS25735 Protein ID WP_001261278.1
Coordinates 23482..23712 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS25720 (18660) 18660..18917 - 258 WP_171929857.1 hypothetical protein -
LOF66_RS25725 (19230) 19230..23024 + 3795 WP_001144732.1 hypothetical protein -
LOF66_RS25730 (23069) 23069..23485 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
LOF66_RS25735 (23482) 23482..23712 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LOF66_RS25740 (24272) 24272..24685 + 414 WP_000465036.1 hypothetical protein -
LOF66_RS25745 (24687) 24687..25466 + 780 WP_001164201.1 site-specific integrase -
LOF66_RS25750 (25645) 25645..26289 + 645 WP_001144036.1 ParA family protein -
LOF66_RS25755 (26376) 26376..26684 + 309 WP_000030199.1 hypothetical protein -
LOF66_RS25760 (27098) 27098..28078 + 981 WP_000688514.1 plasmid segregation protein ParM -
LOF66_RS25765 (28071) 28071..28487 + 417 WP_001278818.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..99606 99606


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T225503 WP_001034046.1 NZ_CP087566:c23485-23069 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T225503 NZ_CP121155:241159-241266 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT225503 WP_001261278.1 NZ_CP087566:c23712-23482 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT225503 NZ_CP121155:c241110-241047 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATAACTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References