Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 23069..23712 | Replicon | plasmid p10_578_2 |
| Accession | NZ_CP087566 | ||
| Organism | Escherichia coli strain CM2010-0578/1 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | C7S9Y5 |
| Locus tag | LOF66_RS25730 | Protein ID | WP_001034046.1 |
| Coordinates | 23069..23485 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | V0SR71 |
| Locus tag | LOF66_RS25735 | Protein ID | WP_001261278.1 |
| Coordinates | 23482..23712 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LOF66_RS25720 (18660) | 18660..18917 | - | 258 | WP_171929857.1 | hypothetical protein | - |
| LOF66_RS25725 (19230) | 19230..23024 | + | 3795 | WP_001144732.1 | hypothetical protein | - |
| LOF66_RS25730 (23069) | 23069..23485 | - | 417 | WP_001034046.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LOF66_RS25735 (23482) | 23482..23712 | - | 231 | WP_001261278.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LOF66_RS25740 (24272) | 24272..24685 | + | 414 | WP_000465036.1 | hypothetical protein | - |
| LOF66_RS25745 (24687) | 24687..25466 | + | 780 | WP_001164201.1 | site-specific integrase | - |
| LOF66_RS25750 (25645) | 25645..26289 | + | 645 | WP_001144036.1 | ParA family protein | - |
| LOF66_RS25755 (26376) | 26376..26684 | + | 309 | WP_000030199.1 | hypothetical protein | - |
| LOF66_RS25760 (27098) | 27098..28078 | + | 981 | WP_000688514.1 | plasmid segregation protein ParM | - |
| LOF66_RS25765 (28071) | 28071..28487 | + | 417 | WP_001278818.1 | plasmid partitioning/stability family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..99606 | 99606 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 14978.31 Da Isoelectric Point: 6.7113
>T225503 WP_001034046.1 NZ_CP087566:c23485-23069 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG
Download Length: 417 bp
>T225503 NZ_CP121155:241159-241266 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8853.07 Da Isoelectric Point: 4.6437
>AT225503 WP_001261278.1 NZ_CP087566:c23712-23482 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL
Download Length: 231 bp
>AT225503 NZ_CP121155:c241110-241047 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATAACTGCAACGTGCGGGGGTTTT
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATAACTGCAACGTGCGGGGGTTTT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9NXF9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SR71 |