225499

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36302..36945 Replicon plasmid p10_578_1
Accession NZ_CP087565
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag LOF66_RS25040 Protein ID WP_001034046.1
Coordinates 36529..36945 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag LOF66_RS25035 Protein ID WP_001261278.1
Coordinates 36302..36532 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS25000 (31515) 31515..31682 + 168 Protein_27 colicin-B -
LOF66_RS25005 (31700) 31700..32227 - 528 WP_000203272.1 colicin B immunity protein -
LOF66_RS25010 (32471) 32471..33286 + 816 WP_032494162.1 lipid II-degrading bacteriocin colicin M -
LOF66_RS25015 (33336) 33336..33690 - 355 Protein_30 colicin M immunity protein -
LOF66_RS25020 (33869) 33869..34660 - 792 WP_000016493.1 site-specific integrase -
LOF66_RS25025 (34657) 34657..35346 - 690 WP_000796228.1 hypothetical protein -
LOF66_RS25030 (35390) 35390..35740 - 351 WP_000493379.1 hypothetical protein -
LOF66_RS25035 (36302) 36302..36532 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LOF66_RS25040 (36529) 36529..36945 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
LOF66_RS25045 (36990) 36990..40784 - 3795 WP_001144732.1 hypothetical protein -
LOF66_RS25050 (41097) 41097..41354 + 258 WP_171929857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN 1..128397 128397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T225499 WP_001034046.1 NZ_CP087565:36529-36945 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T225499 NZ_CP121153:c4725531-4725157 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT225499 WP_001261278.1 NZ_CP087565:36302-36532 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT225499 NZ_CP121153:c4725773-4725531 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References