225498

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4480370..4480988 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LOF66_RS22340 Protein ID WP_001291435.1
Coordinates 4480370..4480588 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LOF66_RS22345 Protein ID WP_000344800.1
Coordinates 4480614..4480988 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS22305 (4475659) 4475659..4476231 + 573 WP_000779831.1 YbaY family lipoprotein -
LOF66_RS22310 (4476262) 4476262..4476573 - 312 WP_000409911.1 MGMT family protein -
LOF66_RS22320 (4476952) 4476952..4477305 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LOF66_RS22325 (4477347) 4477347..4478897 - 1551 WP_268083874.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LOF66_RS22330 (4479061) 4479061..4479531 - 471 WP_000136192.1 YlaC family protein -
LOF66_RS22335 (4479647) 4479647..4480198 - 552 WP_000102564.1 maltose O-acetyltransferase -
LOF66_RS22340 (4480370) 4480370..4480588 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LOF66_RS22345 (4480614) 4480614..4480988 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LOF66_RS22350 (4481534) 4481534..4484683 - 3150 WP_274480386.1 efflux RND transporter permease AcrB -
LOF66_RS22355 (4484706) 4484706..4485899 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T225498 WP_001291435.1 NZ_CP087564:c4480588-4480370 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T225498 NZ_CP121153:c4411061-4410888 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT225498 WP_000344800.1 NZ_CP087564:c4480988-4480614 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT225498 NZ_CP121153:c4411385-4411089 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References