Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3930653..3931229 Replicon chromosome
Accession NZ_CP087564
Organism Escherichia coli strain CM2010-0578/1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LOF66_RS19695 Protein ID WP_001297643.1
Coordinates 3930653..3930940 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LOF66_RS19700 Protein ID WP_000063156.1
Coordinates 3930927..3931229 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOF66_RS19675 (3926906) 3926906..3928186 - 1281 WP_001037380.1 DUF445 domain-containing protein -
LOF66_RS19680 (3928227) 3928227..3929459 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LOF66_RS19685 (3929605) 3929605..3929700 - 96 Protein_3844 dienelactone hydrolase family protein -
LOF66_RS19690 (3929757) 3929757..3930454 + 698 Protein_3845 IS1 family transposase -
LOF66_RS19695 (3930653) 3930653..3930940 + 288 WP_001297643.1 BrnT family toxin Toxin
LOF66_RS19700 (3930927) 3930927..3931229 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LOF66_RS19705 (3931263) 3931263..3932219 - 957 WP_001297640.1 GTPase -
LOF66_RS19710 (3932230) 3932230..3932433 - 204 WP_000467859.1 YbdD/YjiX family protein -
LOF66_RS19715 (3932483) 3932483..3934633 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T225495 WP_001297643.1 NZ_CP087564:3930653-3930940 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T225495 NZ_CP121153:c4199877-4199590 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT225495 WP_000063156.1 NZ_CP087564:3930927-3931229 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT225495 NZ_CP121153:c4199603-4199301 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCCTCGGTGCGTATTGATTCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References